A student planned to utilize HPLC to separate her chemically synthesized product (1) from th reactants (2 and 3).Q1 - Which mode of separation would be reasonable to separate these compounds? Justify your answer HOʻHO`N'H2N1Column: 250 x 30 mm C18 10u 100ÅFlow: 5 mL/minOven temperature: 25 oCMobile Phase: A = water,Gradient: at 0 min 20 %Bat 5 min 20 %Bat 15 min 100 %Bat 20 min 20 %BB = 90% Acetonitrile/WaterInjection volume: 5 uL%3D2.

HPLC stands for High Performance Liquid Chromatography which is used to separate and identify…

A student planned to utilize HPLC to separate her chemically synthesized product (1) from th reactants (2 and 3). Q1 - Which mode of separation would be reasonable to separate these compounds? Justify your answer

A student planned to utilize HPLC to separate her chemically synthesized product (1) from th reactants (2 and 3). Q1 - Which mode of separation would be reasonable to separate these compounds? Justify your answer

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter26: An Introduction To Chromatographic Separations

Section: Chapter Questions

Problem 26.1QAP

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Concept explainers

Mass Spectrometry

This process is an instrumental method in which a sample is changed to fast moving positive ions by electron bombardment. The charged particles vary due to their different mass to charge ratio and are estimated with the help of this difference. It is also called mass spectroscopy.

Question

A student planned to utilize HPLC to separate her chemically synthesized product (1) from th reactants (2 and 3).

Q1 - Which mode of separation would be reasonable to separate these compounds? Justify your answer

HOʻ
HO
`N'
H2N
1
Column: 250 x 30 mm C18 10u 100Å
Flow: 5 mL/min
Oven temperature: 25 oC
Mobile Phase: A = water,
Gradient: at 0 min 20 %B
at 5 min 20 %B
at 15 min 100 %B
at 20 min 20 %B
B = 90% Acetonitrile/Water
Injection volume: 5 uL
%3D
2.

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