BiochemistryADDITIONAL QUESTIONS1. A technician checks the column bed volume on a column that is supposed to be 2ml. If the bed volume is not 2ml, isthis a problem? Why or why not?2. The column drips very slowly. Suggest a method to increase the rate of flow through the column. How can you checkto ensure that this method does not compromise the separation?3. A scientist would like to know the charge of a certain protein at pH 7.2. How could this column be used to helpdetermine the overall charge of the protein at pH 7.2? What problems might occur using the proposed approach,and how might they be addressed?Inet eavad: 6/19/2017 CMH

Column chromatography is a separation and purification technique. Column chromatography requires a…

Answered: A technician checks the column bed…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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