An enzyme catalyzes a reaction with a Km of 8.50 mM and a Vmax of 3.45 mM · s-. Calculate the reaction velocity, vo, for each substrate concentration. [S] = 1.25 mM %D Vo : mM · s-1

Q: An enzyme catalyzes a reaction at a velocity of 20 micromole/min when the concentration of substrate…

A: The Michaelis-Menten equation represents the relationship between substrate concentration and…

Q: Calculate kcat for an enzyme where Vmax is 12 U s. mL and the total enzyme concentration is 0.016 U…

A: Given Values: Vmax = 12 u s-1ml-1 Total enzyme concentration = 0.016 U ml-1

Q: An enzyme that follows simple Michaelis–Menten kinetics has an initial reaction velocity of 10…

A: The answer is given in the image given below.

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Q: The following data describe an enzyme-catalyzed reaction. Plot these results using the…

A: Enzyme catalyzed reactions follow Michaelis-Menten equation. V=Vmax [S]Km+[S] Here, Velocity of the…

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Q: Explain why each of the following data sets from a Lineweaver–Burk plot are not individually ideal…

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Q: [S] mM 20 50 100 200 300 500 700 Initial Velocity (mM/min) 1.012 1.950 2.823 3.637 4.024 4.398 4.580

A: Enzyme kinetics can  be determined by constructing mm plot or lb plot. With them km and Vmax can be…

Q: Vmax and Km were calculated for enzyme substrate data. Results Y=0,0063x+0,3929 Km =62,4 mikromol/L…

A: From the LB plot of enzyme kinetics, y=mx+c  It's an equation of a straight line. 1/V=(Km/Vm )1/S…

Q: 8- 2- 315 405 [Substrate] (nM) 45 90 135 180 225 270 360 450 495 540 58! What is the Vmax of the…

A: Michaelis Menton kinetics: This is well known model for Enzyme kinetics. This model relates rate of…

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Q: Most of the enzyme reactions followed the mathematical kinetic plots suggested by Michaelis-Menten…

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Q: when saturated with substrate, an enzyme has a maximum initial rate of 110mumoles of substrate…

A: Asked : Reaction rate when substrate concentration is 2x10^-3M

Q: 1. Calculate the reaction Kcat for the Control in experiment (1). 2. Draw a velocity versus [S]…

A: Michaelis Menton equation relates velocity of enzymatic reaction with substrate concentration.…

Q: If a michaelis-type enzyme has an apparent Km of 2*10^-8 and apparent Vmax of 1*10^-4 moles/min in…

A: Uncompetitive inhibitors bind only to the enzyme–substrate complex and reduce both kcat and Km (the…

Q: An enzyme is present at 100 nM (nanomolar) and has a Vmax value of 25 uM/s (micromolar/second). The…

A: Michaelis–Menten kinetics is a well established model of enzyme kinetics.  The Michaelis -Menten…

Q: Lineweaver-Burk plots of enzyme kinetics for the reaction, S P, has the following features: 1/v is…

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Q: The following data were obtained for a reaction that catalyzed by an enzyme: Initial concentration…

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A: Enzymes are biological catalysts that help chemical processes occur faster. Enzymes are proteins…

Q: [S, mmol L-') Vo (umol L-' min-') 0.5 50 1.0 2.0 3.0 10.0 8||||

A: Given values; Km of the enzyme = 1 mM At S1= 0.5 mM, Vo = 50 μmol/min S2 = 1 mM, Vo=? S3 = 2 mM ,…

Q: When [Et] = 20 nM and [SAD] = 40 µM, the reaction velocity, V0, is 9.6µM s1. Calculate Km for the…

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Q: L(24 points) Explain How is the Michaelis constant defined. and what does a low or high value for Km…

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Q: calculate the reaction velocity at saturating substrate concentrations. Your numerical answer is…

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Q: In a 250ul reaction containing 0.3 nmol of a Michaelis enzyme with Km = 4.4 x 10 M and saturating…

A: Given, Vo= 6.3 * 10-3 M min-1 Km= 4.4 * 10-5 M [S]= 1 (Because its given that the condition is…

Q: concentration of each enzyme and with [X] = 1 µM. Which curve corresponds to which enzyme? Explain.

A: Given,  the substrate concentration is [X] =1 μM Km of enzyme A = 2.0 μM Km of enzyme B = 0.5 μM

Q: Inside cells, the AG value for the hydrolysis of ATP to ADP + Pi is approximately -50 kJ/mol (-12…

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Q: Of the following constants in Enzyme kinetics, this is catalytic efficiency: О а. Vmax O b. Kcat/Km…

A: Introduction: Enzymes are proteins that increase the rate of the reaction that occurs in the living…

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A: We know by Michaelis–Menten kinetics:  V0= Vmax[S]/ Km +[S] V0= velocity of reaction Vmax= maximum…

Q: Vo with Vo without [Substrate, µM] DEDS DEDS (µM/min) (µM/min) 3.333 0.774 1.196

A: Enzymes are protein molecules that increase the rate of reaction by decreasing the activation energy…

Q: You measure the kinetics of an enzyme as a function of substrate concentration using the…

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Q: The following results were obtained for an enzyme-catalysed reaction Substrate concentration (mmol…

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Q: For some Enzyme, the Vmax is 18 micromols/min, Km is 400 microM. If the substrate concentration is…

A: Velocity of the reaction defines the rate of reaction which is catalyzed by the enzyme. It depends…

Q: An enzyme that follows simple Michaelis–Menten kinetics has an initial reaction velocity of 10…

A: GIVEN VALUES: Initial velocity= 10 μmol.min-1 Substrate concentration is 5 time the value of Km .…

Q: If the enzyme lactase has a Vo of 0.40 mM per minute when [S] = 1.25 mM, and a Vo of 1.0 mM per…

A: If an enzyme follows Michaelis-Menten Kinetics, a plot of the reciprocal of the reaction velocity…

Q: enzyme converts substrate to product at a rate of 0.010 mmoles/sec. Assuming that Michaelis- Menten…

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Q: 1. Enzyme X has a molecular weight of 35,000 Da. When this enzyme is assayed for enzyme activity,…

A: Enzyme kinetics is used to study the rates of enzyme catalysed biochemical reactions. In this study…

Q: Consider the following reaction:ATP → AMP + 2 PiCalculate the equilibrium constant (Keq) given the…

A: The energy currency of the living cell is called ATP. It is found in every organism. It is utilized…

Q: The following data were obtained in a study of an enzyme known to follow Michaelis-Menten Kinetics.…

A: In-order to get an accurate value of KM , we need to plot the Lineweaver Burk (LB) plot. The X and Y…

Q: Use the Michaelis-Menten equation to complete the enzyme kinetic data set, when Km is known to have…

A: The Michaelis-Menten equation can be written as: V0=Vmax × [S]Km + [S] From the question it is given…

Q: A bacterial enzyme catalyzes the hydrolysis of maltose as shown in the reaction given below:…

A: the enzyme kinectics is governed entirely by one equation, the Michaelis-Menten Equation. the…

Q: Consider the following data for an enzyme-catalyzedhydrolysis reaction in the presence and absence…

A: The reciprocal of the given data is as follows: 1/S (Mol-1) 1/V (min/micromol) Uninhibited 1/V…

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Q: Use the Michaelis-Menten equation to complete the enzyme kinetic data set, when Km is known to have…

A: Introduction:  The theory of enzyme kinetics was proposed by two scientists Leonor Michaelis and…

Q: You measure the kinetics of an enzyme as a function of substrate concentration using the…

A: Enzyme kinetics is used to study the rates of enzyme catalyzed biochemical reactions. In this study…

Q: 1.2) If the Km of an enzyme for it's substrate remains constant as the concentration of the…

A: If Km remains unchanged even after increasing the inhibitor concentration it shows that…