Assume that you used malate dehydrogenase as the starting material for engineering this enzyme. Explain in detail what changes you would have to make to malate dehydrogenase for it to catalyze this new reaction instead of its usual reaction.
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- Using the ActiveModel for enoyl-CoA dehydratase, give an example of a case in which conserved residues in slightly different positions can change the catalytic rate of reaction.The Reactions and Meehanisms of the Leloir Pathway Write the reactions that permit galactose to be utilized in glycolysis. Write a suitable mechanism, tor one of these reactions.Examine the ActiveModel for alcohol dehydrogenase and describe the structure and function of the catalytic zinc center.
- Extending the Mechanism of Methylmalonyl-CoA Mutase to Similar Reactions Based on the mechanism for the methylmalonyl-CoA mutase (see problem 14), write reasonable mechanisms for the following reactions shown.Alcohol dehydrogenase catalyzes the oxidation of an alcohol to the next highest state of oxidation, an aldehyde. How does this partially explain the toxicity of methanol?Explain how the conversion of alanine to pyruvic acid (pyruvate) can be identified as an oxidation reaction.
- Mercuric ion and methanol are inhibitors of alcohol dehydrogenase. Explain.The uncatalyzed reaction rate for the conversion of substrate X to product Y is one year. The enzyme-catalyzedrate is one millisecond. Describe the features of theenzyme that are probably responsible for this ratedifference.Suggest a name for an enzyme that catalyzes each of the following reactions. Oxidation of nitrite Decarboxylation of citrate Reduction of oxalate
- In some brain cancer cells, a mutated form of isocitrate dehydrogenase, instead of catalyzing the oxidation of the secondary alcohol of isocitrate, catalyzes the reduction of a-ketoglutarate. Draw the product of the reaction.What is alpha keto glutarate dehydrogenase complex?. explain very briefly.Suggest a name for an enzymes that catalyzes each of the following reactions.1. Hydrolysis of Lactose 2. Oxidation of Nitrate3. Decarboxylation of Citrate4. Reduction of Exalate