In the DNA extraction. What is the role of alcohol in the DNA extraction process?
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In the DNA extraction.
What is the role of alcohol in the DNA extraction process?
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- Single-strand binding proteins keep the two parental strands of DNA separated from each other until DNA polymerase has an opportunity to replicate the strands. Suggest how single-strand binding proteins keep the strands separated and yet do not impede the ability of DNA polymerase to replicate the strands.Replication:- what other enzymes are involved in the initiation phase?- explain the role of primers in this phase- how is the building of the leading strand different from that of the lagging strand?Which of these two changes is more difficult for DNA repair enzymes to fix correctly? Explain why.
- DNA Profiles as Tools for Identification A PCR-based paternity test is conducted using STRs that consistently produce a unique DNA fragment pattern from a single chromosome. Examining the results of the following Southern blot, which male(s) can be excluded as the father of the child? Which male(s) could be the father of the child?Explain why base excision repair, nucleotide excision repair, and mismatch repair—which all require nucleases to excise damaged DNA—require DNA ligase.True or false? DNA repair mechanisms all depend on the existence of two copies of the genetic information, one in each of the homologous chromosomes. DNA primase makes DNA primers for the synthesis of Okazaki fragments in the DNA lagging strand. The repair of double-stranded breaks by nonhomologous end joining is accurate. RNA polymerase III transcribes all protein-coding genes.
- Explain the molecular mechanism of DNA polymerization by DNA polymerase and explain why DNA polymerase 3 and not dna polymerase 1 is responsible for replicating the bacterial genome.Describe the function of DNA polymerase. Explain why each part of the name DNA polymerase (DNA, polymer, -ase) makes sense.True or false: The synthesis of a new strand of DNA is initiated using a DNA primer?
