CAR) All une Red stock soluton concentration: Somple #tck AR/ vaume dilution 1 valume total AR Coantratin Abserbonce 50.00m4 50. 00mL 50.00 L So00 O. 16.4 O. 237 O. 392 10 dilution 3 20 dilution 4 25 312 unknarn - 0.485 Incude a printed copy of the colibration curve) Lolibrotion curve cayotion: R valve
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- Suppose you have a concentration of 356ng/uL for Sample A. How many ng of protein will that be?The organophosphate Azinphos-methyl has a96-hour LC50 value of 3 ppb for rainbow trout. Inan unfortunate incident, 200 g of this pesticidewas sprayed on a field, and a subsequent heavyrainfall washed 35% of it into a nearby lakehaving a surface area of 30,000 m2and anaverage depth of 0.5 m. Would the pesticideconcentration in the lake water have beensufficient to kill a significant fraction of therainbow trout in the lake?1.2. Using DEAE-cellulose as ion exchange resin, indicate the starting and ending pH for the narrowest experimental pH range used to separate an amino acid mixture consisting of Cys, His and Leu Starting pH: _____ Ending pH: _____
- If I had a standard with 2.0 mL of Bradford reagent then added 50μL of 250 μL/mL ; what would be the concentration of original protein?briefly explain a. K-Mer b. B ion c. HDRConsider the titration of 30.0 mL of 0.0700 M HONH2 (a weak base; Kb = 1.10e-08) with 0.100 M HI. Calculate the pH after the following volumes of titrant have been added: (a) 0.0 mLpH = (b) 5.3 mLpH = (c) 10.5 mLpH = (d) 15.8 mLpH = (e) 21.0 mLpH = (f) 27.3 mLpH =
- Calculate the amount of protein (in mg) in Sample 1 if the measurement at A280 = 0.636, taking into account the dilution factor as per question 6 (100ul) and the total volume of extract as per question 4 (140ml). Give your answer as a number with no decimal places.I try to prepare for my biological chemistry exam, so I will appreciate if the answer is with some details. And I will be happy if there would be explanation for when I should use wich of the pka's. Thank you so much.What's the amount of protein if the measurement at A280 = 1.120, dilution factor is 100 and total volume of extract is 45
- What's mendes law4,5,6 please answer all give the significance/role/effect of the reagent/condition in the isolation or analysis of a biomolecule. limit answers to two short sentencesCalculate protein concentration in unknown samples 1, 2, 3: Absorbance of Unknown 1 = 0.541 Absorbance of Unknown 2 = 0.85 Absorbance of Unknown 3 = 1.02 Standard Curve: Y = 0.0073x