Chapter 21 describes two blotting methods (i.e., Northern blotting and Western blotting) used to detect gene products. Northern blotting detects RNA and Western blotting detects proteins. Suppose that a female fruit fly is heterozygous for a maternal effect gene, which we will call gene B. The female is Bb. The normal allele, B, encodes a functional mRNA that is 550 nucleotides long. A recessive allele, b, encodes a shorter mRNA that is 375 nucleotides long. (Allele b is due to a deletion within this gene.) How could you use one or more of these techniques to show that nurse cells transfer gene products from gene B to developing oocytes? You may assume that you can dissect the ovaries of fruit flies and isolate oocytes separately from nurse cells. In your answer, describe your expected results.
5. Chapter 21 describes two blotting methods (i.e., Northern blotting
and Western blotting) used to detect gene products. Northern blotting
detects RNA and Western blotting detects proteins. Suppose
that a female fruit fly is heterozygous for a maternal effect gene,
which we will call gene B. The female is Bb. The normal allele, B,
encodes a functional mRNA that is 550
allele, b, encodes a shorter mRNA that is 375 nucleotides
long. (Allele b is due to a deletion within this gene.) How could
you use one or more of these techniques to show that nurse cells
transfer gene products from gene B to developing oocytes? You
may assume that you can dissect the ovaries of fruit flies and isolate
oocytes separately from nurse cells. In your answer, describe
your expected results.
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