Consider the proteins in Figure 1. Assume they are treated with chymotrypsin to cut them into fragments, and then the fragments are separated by gel electrophoresis. What would the fragment patterns look like in the gel for the three different proteins, assuming we can separate polypeptides that differ in size by very small amounts? Complete Figure 2 below to show the location of the uncut polypeptides (- enzyme lanes) and the cut fragments (+ enzyme lanes). Protein 1Protein 2Protein 3+++WellsFigure 2: Gel electrophoresis of proteins from Figure 1 (- enzyme lanes) as well as cutfragments from those proteins generated through treatment by chymotrypsin (+ enzymelanes). The location of the band for the uncut protein 1 is shown. Protein 1: yal glu glu gln arg tuR val leu ala his thr gln arg glu gln met val alaProtein 2: yal glu glu gln arg trp val leu ala his thr gln phe'glu gln met val alaProtein 3: yal thr gln phe glu leu arg glu trp xal ala met gln phe gln glu val alaFigure 1: The amino acid sequences for three small proteins. Arrows mark the potentialcut sites for the enzyme chymotrypsin.

Chymotrypsin: It is a proteolytic enzyme that cuts the protein at a specific point. Proteolytic…

Consider the proteins in Figure 1. Assume they are treated with chymotrypsin to cut them into fragments, and then the fragments are separated by gel electrophoresis. What would the fragment patterns look like in the gel for the three different proteins, assuming we can separate polypeptides that differ in size by very small amounts? Complete Figure 2 below to show the location of the uncut polypeptides (- enzyme lanes) and the cut fragments (+ enzyme lanes).

Consider the proteins in Figure 1. Assume they are treated with chymotrypsin to cut them into fragments, and then the fragments are separated by gel electrophoresis. What would the fragment patterns look like in the gel for the three different proteins, assuming we can separate polypeptides that differ in size by very small amounts? Complete Figure 2 below to show the location of the uncut polypeptides (- enzyme lanes) and the cut fragments (+ enzyme lanes).

Nutrition Now

8th Edition

ISBN:9781337415408

Author:Brown

Publisher:Brown

Chapter15: Proteins And Amino Acids

Section: Chapter Questions

Problem 12RQ

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