Consider the pyruvate carboxylase reaction, the first bypass step in gluconeogenesis: Pyruvate + CO₂ + ATP = Oxaloacetate + ADP + P₂ Equilibrium concentrations were measured as follows: [pyruvate] =0.451 mM [HCO;] -7.02 mM (note that pCO₂ is represented as [HCO;']) [ATP] = 0.375 mM [oxaloacetate] =0.671 mM [ADP] = 0.963 mM [P] = 11.0 mM a) From the above data, calculate Ke, for the pyruvate carboxylase reaction b) Calculate AG" for this reaction c) Calculate AG under the following conditions: • [pyruvate]- 1.24 mM • [HCO3] = 7.69 mM [ATP] = 1.4 mM [oxaloacetate] =0.001 mM • [ADP]=0.032 mM [P] = 10.0 mm

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
icon
Related questions
Question
100%

My teacher did not go over this really well in class so i'm looking for extra help.

Consider the pyruvate carboxylase reaction, the first bypass step in gluconeogenesis:
Pyruvate + CO₂+ ATP Oxaloacetate + ADP + Pi
Equilibrium concentrations were measured as follows:
[pyruvate] = 0.451 mM
[HCO3] = 7.02 mM (note that pCO₂ is represented as [HCO3])
[ATP] = 0.375 mM
[oxaloacetate] = 0.671 mM
●
•
[ADP] = 0.963 mM
[Pi] = 11.0 mM
a) From the above data, calculate Keq for the pyruvate carboxylase reaction
•
●
b) Calculate AG for this reaction
c) Calculate AG under the following conditions:
· [pyruvate] = 1.24 mM
[HCO3] = 7.69 mM
[ATP] = 1.4 mM
[oxaloacetate] = 0.001 mM
[ADP] = 0.032 mM
[P] = 10.0 mM
●
C
ZOOM
+
Transcribed Image Text:Consider the pyruvate carboxylase reaction, the first bypass step in gluconeogenesis: Pyruvate + CO₂+ ATP Oxaloacetate + ADP + Pi Equilibrium concentrations were measured as follows: [pyruvate] = 0.451 mM [HCO3] = 7.02 mM (note that pCO₂ is represented as [HCO3]) [ATP] = 0.375 mM [oxaloacetate] = 0.671 mM ● • [ADP] = 0.963 mM [Pi] = 11.0 mM a) From the above data, calculate Keq for the pyruvate carboxylase reaction • ● b) Calculate AG for this reaction c) Calculate AG under the following conditions: · [pyruvate] = 1.24 mM [HCO3] = 7.69 mM [ATP] = 1.4 mM [oxaloacetate] = 0.001 mM [ADP] = 0.032 mM [P] = 10.0 mM ● C ZOOM +
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 4 steps

Blurred answer
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON