cytosol but those without this substance have concentrations of up to 50uM in the cytosol by the time they die.  What does this tell you about the function of the isolated substance, OmpF, which you suspected from the start was related to quinolone resistance?  How is the OmpF structure optimized for this function? Which amino acids in OmpF are suited to each aspect of its function? The structure of ciprofloxacin is shown below for reference.

Biochemistry
6th Edition
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Reginald H. Garrett, Charles M. Grisham
Chapter32: The Reception And Transmission Of Extracellular Information
Section: Chapter Questions
Problem 1P
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Suppose you discover that bacteria that express OmpF have concentrations of <0.1uM of ciprofloxacin in the cytosol but those without this substance have concentrations of up to 50uM in the cytosol by the time they die.  What does this tell you about the function of the isolated substance, OmpF, which you suspected from the start was related to quinolone resistance?  How is the OmpF structure optimized for this function? Which amino acids in OmpF are suited to each aspect of its function? The structure of ciprofloxacin is shown below for reference.  

 

 

 

HN
N.
HO
F
Ciprofloxacin
Transcribed Image Text:HN N. HO F Ciprofloxacin
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