Describe the mechanism of a-chymotrypsin. Explain the roles of constituents of the catalytic triad, their modes of catalysis, and the significance of the oxyanion hole in the catalysis.
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- One treatment for hyperuricemia is administration of xanthine oxidase inhibitors like allopurinol. What is the biochemical rationale for this treatment? Discuss the mechanism and show an illustration how this drug able to alleviate symptoms of hyperuricemia?Most individuals with genetic defects in oxidative phosphorylation are found to have relatively high concentrations of alanine in their blood. how this in biochemical terms? please help :)Explain the observation made when papase is added to the reaction catalyzed polyphenoloxidase?
- Using the principles described in the text regarding pyridoxal phosphate mechanisms, propose a mechanism for the reaction catalyzed by serine hydroxymethyltransferase.Explain the role and functioning of chaperone proteins. Explain, why several chaperones (chaperonins) possess an ATPase activity?Briefly describe the role of nucleophilic catalysis in the mechanism of the chymotrypsin reaction.
- Explain the Phosphorylation of glycogen phosphorylase by phosphorylase kinase mechanism. What is happening in each step? what are activating nucleophiles and stabilizing intermediates?Only five general types of reactions are commonly observed in biochemical transformations. Given the proposed mechanism (illustrated above) for Class A β-lactamases, what is the biochemical reaction type represented by steps 1 and 2?In working skeletal muscle under anaerobic conditions, glyceraldehyde 3-phosphate is converted to pyruvate (the payoff phase of glycolysis), and the pyruvate is reduced to lactate. Write balanced biochemical equations for all the reactions in this process, with the standard free-energy change for eachreaction. Then write the overall or net equation for the payoff phase of glycolysis (with lactate as the end product), including the net standard free-energy change.