Fumarase catalyzes the hydration of fumarate to L-malate via the following reaction: Fumarate + H2O → L-Malate Following data below were obtained when fumarate was used as a substrate and the initial rates of hydration were measured at pH 5.7 and 25°C with an enzyme concentration of 2 x10-6M. Plot the data in Lineweaver-Burke form and determine Vmax and Km for fumarase under these conditions.

Fumarase catalyzes the hydration of fumarate to L-malate via the following reaction: Fumarate + H2O → L-Malate Following data below were obtained when fumarate was used as a substrate and the initial rates of hydration were measured at pH 5.7 and 25°C with an enzyme concentration of 2 x10-6M. Plot the data in Lineweaver-Burke form and determine Vmax and Km for fumarase under these conditions.

Principles of Modern Chemistry

8th Edition

ISBN:9781305079113

Author:David W. Oxtoby, H. Pat Gillis, Laurie J. Butler

Publisher:David W. Oxtoby, H. Pat Gillis, Laurie J. Butler

Chapter11: Solutions

Section: Chapter Questions

Problem 9P

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