Answered: Given the following data for an…

Given the following data for an enzyme-catalyzed reaction. Substrate conc., mM Velocity, mM/s 0.10 0.96 o.125 1.12 0.167 1.35 0.250 1.66 0.50 2.22 1.00 2.63

Fundamentals Of Analytical Chemistry

9th Edition

ISBN:9781285640686

Author:Skoog

Publisher:Skoog

Chapter12: Gravimetric Methods Of Analysis

Section: Chapter Questions

Problem 12.2QAP

See similar textbooks

Related questions

Q: Subtilisin is a protease that is often added to laundry detergents to help remove protein-based…

Q: Assuming an excess of substrate, if the enzyme concentration is doubled in a reaction vessel, the…

A: Applying concept of enzyme catalysis.

Q: The catalytic activity of an insect aminopeptidase was investigatedusing an artificial peptide…

A: Enzymes are known as bio-catalyst. They enhance the reactions taking place inside our body. Enzyme…

Q: If the enzyme maltase has a Vo of 0.25 mM per minute when [S] = 0.10 mM, and a Vo of 0.40 mM per…

A: The Vmax for the reaction in accordance with the Michaelis-Menten equation calculated as shown below…

Q: Imagine you need to pipet 0.15 mL of ONPG stock solution into an enzyme assay. What volume is this…

A: The given volume of ONPG stock solution is 0.15 mL.

Q: An enzyme whose KM is 10-4 M in the presence of a substrate concentration of 10-2 M It is capable of…

Q: The isoelectric point, pI, of the protein horse liver alcohol dehydrogenase is 6.8 , while that of…

A: Since you have posted a question with multiple questions, we will solve first question for you. To…

Q: Calculate the value of the denaturation constant kd for a given enzyme, using the equation provided…

Q: As you increase the temperature of an enzyme-catalyzedreaction, the rate of the reaction initially…

Q: Given the following information, calculate the catalytic efficiency of the enzyme (numerical answer,…

A: Given that - [S] = 100 mM k1 = 10 sec-1 k2 = 3000 sec-1 k-1 = 20 sec-1 [E]T = 1 \muμM The…

Q: 3. The initial rates, v, for the breakdown of glutamic acid by glutamate deghydrogenase are as…

A: Introduction: The rate of reaction (r) for enzyme catalytic reaction is the function of the…

Q: A low value of the Michalis constant (KM) in an enzyme-catalyzed reaction indicates--- The enzyme…

A: Km is the Michaelis-Menten constant which shows the concentration of the substrate when the reaction…

Q: Which of the following tetrapeptides will be retained on a cation-exchange column at pH 8.2? O HGTH…

A: Ion exchange chromatography separates ions and molecules based on their net overall surface charge.…

Q: WHAT IS THE UNCATALYZED NEUTRAL REACTION IN WATER (need to have substrate, product, and qualitative…

Q: From the given activity, 1.) What is the effect of temperature to the enzyme? 2.) What are some…

A: Hi, we are supposed to answer one question. To get the remaining questions solved please mention the…

Q: Concentration, uM Vo (umol/min) w/o Vo (umol/min) with substance B substance B 0.4 0.08 0.069 0.7…

Q: Some proteases are cysteine proteases and threonine proteases as opposed to serine proteases. What…

A: Given proteases, Cysteine proteases Threonine proteases Serine…

Q: What is responsible for the large range in values of dissociation constants listed in Table 2.18?

A: In the above given types of bio affinity interactions there are different types of intermolecular…

Q: Given the following peptide SEPIMAPVEYPK(a)…

Q: A substitution of the amino acid phenylalanine for glycine at the active site of an enzyme would…

A: Answer

Q: How would changing the enzyme concentration affect Km and Vmax

A: In Enzyme catalysis, the enzyme is used as the catalyst to increase the rate of the reaction without…

Q: Ah enzyme interhational the quantity of enzyme heeded to Pedct With substrate per minute. 1 mole lu…

A: Enzymes create chemical reactions in the body, actually, these enzymes act like a catalyst and…

Q: Please answer all 8 blanks please!

A: Enzymes are basically proteins. These are substances that acts as a catalyst in living organisms,…

Q: Temperature 4 °C 21 °C 30 °C 37 °C 90 °C Absorbance at zero min 1.300 0.952 0.876 0.914 0.903…

A: As per Beer-Lambert's law, A=εbcwhere,A=Absorbanceε=Extinction coefficient (in M-1.cm-1)b=path…

Q: Suppose that Vmax for 1 mg/ml of a pure enzyme is 30 mM/min, i.e., Vmax is 30 (umols/ml)•min. The…

Q: A plot of activity versus pH for an enzyme that functions in the cytosol of a cell at pH of about 7…

A: The activity of enzymes varies at different pH levels.

Q: 15.) On a Lineweaver-Burk plot, a changing y-intercept upon addition of increasing concentration of…

A: Enzymes are the specific catalyst that is used in biochemical reactions. The kinetics of enzymes is…

Q: Identify the kind of chemical catalysis and then identify which of the given amino acids can act as…

A: Concept and Reason The concept used here is an enzyme catalysis. The enzyme enhances rate of…

Q: The disease Phenylketonuria (or PKU) occurs when the enzyme that helps to catalyze the conversion of…

A: Hello. Since your question has multiple parts, we will solve the first question for you. If you want…

Q: The protein catalase catalyzes the reaction 2H,0, (aq) – 2 H,O(0) + 0,(g) and has a Michaelis-Menten…

Q: -1 An uncatalyzed metabolic reaction has a rate constant of 2.7 x 10-4 M-¹ min-¹ at 310 K. In the…

A: The minimum amount of energy required by the reactant to cross the energy barrier to give the…

Q: Which of the following is true for enzyme kinetics? A Only Vmax can be calculated from the…

A: Interpretation - To tell about which one is true for the enzyme kinetics - Introduction -…

Q: Assume that an enzyme catalyzed reaction follows the scheme shown: k2 k1 ES E + P E + S k.1 Where k,…

Q: Please answer C and D.

A: C)Equation for Vmax

Q: Determine the values of KM and Vmax. For the Vmax obtained, calculate the turnover number assuming…

A: The graph formed will be as follows: According to the Michaelis Menten equation where R is the…

Q: The recommended working buffer for the restriction enzyme Notl is compe 10 mM MgCh 100 mM NACI 50 mM…

A: Tris HCl is an organic compound often used in buffer solutions such as TAE or TBE for…

Q: a) CH3COSCOA + CO2 e) COCI2 SH Biotin, enzyme HO. 1) NaH, THF 2) excess PhMgBr Ph Ph NOH…

A: Since you have posted a question with multiple sub-parts, we will solve the first three subparts for…

Q: 1. Using the equation E + S ⇆ ES → E + P, supply reaction involved in the experiment... 2. How did…

A: A question based on catalysis that is to be accomplished.

Q: Salicylate (aspirin) inhibits the catalytic action of glutamate dehydrogenase. You measure the…

A: An enzymatic reaction is similar to catalyzed chemical reaction. An enzyme acts a catalyst in the…

Q: 17.) In the left graph below, the dashed line (- - - -) represents what? В) Km A) Vmax C)…

A: Vmax is the maximum rate of reaction when enzyme reacts with substrate and Km is the Michael's…

Q: A certain enzyme has a glutamic acid residue in its active site that is responsible for the enzyme's…

A: The explanation is given below-

Q: KM and Vmax- тах.

A: The graph formed will be as follows: According to the Michaelis Menten equation

Q: The rate of an enzyme-catalyzed reaction (in mM/min) was determined at different concentrations of…

A: Since you have asked multiple questions, we will solve first question (Q.No.-14) for you. For…

Q: Plots A and B show enzyme kinetics data in the absence and presence of inhibitor. Which response…

A: A question based on kinetics of reactions in solution that is to be accomplished.

Q: Among these protein assays, which work in acidic conditions? a. Biuret assay b. Bicinchoninic acid…

A: Biuret assay works in alkaline medium, with the help of sodium potassium tartrate, it react with…

Q: 460 410 360 310 260 210 160 110 60 -10 -510 4 5 10 15 20 25 30 35 40 45 50 55 1/[S] per mM 1. What…

A: Michaelis Menten equation shows the effect of the concentration of the substrate on the rate of an…

Q: 5. Varying the enzyme- For a one substrate, enzyme-catalyzed reaction, double reciprocal plots were…

A: The Vmax (maximum rate or velocity) directly related to the concentration of the enzyme, [E], means…

Question

Given the following data for an enzyme-catalyzed reaction.

Substrate conc., mM	Velocity, mM/s
0.10	0.96
o.125	1.12
0.167	1.35
0.250	1.66
0.50	2.22
1.00	2.63

1.Write the equation of the line consistent with the Lineweaver- Burke equation (type answer in this format: y=mx+b)

2. Calculate the Vmax.

3. Calculate the Km.

Please help me. Kindly explain step by step the solution to this problem. Thank you.

Expert Solution

This question has been solved!

Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.

SEE SOLUTION Check out a sample Q&A here

Step 1

VIEW

Step 2

VIEW

Step by step

Solved in 2 steps

SEE SOLUTION Check out a sample Q&A here

Similar questions

An enzyme-catalyzed reaction has a Km of 1.4 mM and a Vmax of 7 nM/s. What is the initial velocity when the substrate concentration is 0.6 mM?
An enzyme-catalyzed reaction has a Km of 1 mM and a Vmax of 4 nmole/L sec-1. The reaction velocity (nmole/L sec-1) when the substrate concentration is 0.25 mM is: A. 1.25 B. 10.0 C. 5.0 D. 0.50 E. 1.0 F. 100 G. 150 H. 55 I. 75
Enzyme X can act on either substrate A or B; the Km on A < Km on B. Vmax for A > Vmax on B.Sketch 1 plot for 1/v vs. 1/[S] plot for Enzyme X in the presence of A and in the presence of B.
Your body deals with excess nitrogen by excreting it in the form of urea, NH2CONH2. The reaction producing it is the combination of arginine (C6H14N4O2) with water to give urea and ornithine (C5H12N2O2). C6H14N4O2 + H2O ? NH2CONH2 + C5H12N2O2 [Molar masses: 174.2 18.02 60.06 132.2] If you excrete 95 mg of urea, what quantity of arginine must have been used? What quantity of ornithine must have been produced?
Determine the value of the turnover number of the enzyme catalase, given that RmaxRmax (Vmax)(Vmax) for catalase is 41 mmol⋅L−1⋅s−141 mmol⋅L−1⋅s−1 and [E]t[E]t equals 4.8 nmol⋅L−14.8 nmol⋅L−1 . Catalase has a single active site.
The enzyme urease increases the rate of urea hydrolysis at pH 8.0pH 8.0 and 20 °C20 °C by a factor of 1014.1014. Suppose that a given quantity of urease can completely hydrolyze a given quantity of urea in 88 minutes at pH 8.0pH 8.0 and 20 °C.20 °C. How long would it take for this amount of urea to be hydrolyzed in the absence of urease at the same temperature and pH in sterile conditions? how many years?
A An enzyme that follows Michaelis-Menten kinetics has a KM value of 16.0 uM and a kcat value of 181 s-1. At an initial enzyme concentration of 0.0100 uM, the initial reaction velocity was found to be 1.07 x 10- uM/s. What was the initial concentration of the substrate, [S], used in the reaction ? Express your answer in micromolar to three significant figures
Given the Ksp for Ag2CrO4 is equal to 1.20 x 10-12, calculate the percent error in your experimentally determined value for average Ksp (this will be an extremely large percentage – think about why).
18. Jet is an undergraduate chemistry student, he’s out in the laboratory trying to determine the volatile organic compounds as well as overall protein content of the leaf and stem of a malunggay (Moringa oleifera). He subjected the leaf and stem in a separate digestion reaction (treatment of sulfuric acid), afterwards he subjected the products to high temperature induction to get a dry ash like substance. Which type/s of chemical analysis did Jet employ to reach his objective?I. Qualitative AnalysisII. Quantitative Analysis III. Instrumental Chemical AnalysisIV. Wet Chemical AnalysisA. II & IV onlyB. I & IV onlyC. I, II & IV onlyD. I, II, III, IV
Three large proteins are ionized at the pH at which an electrical FFF separation is carried out. (i) if the ions are designated A2+, B2+, and C3+, predict the order of elution. (ii) What is FFF? (iii) List the four subtechniques of FFF.
Given an enzyme with KM of 0.5mM, at what substrate concentration will the velocity of the enzyme reach ¼ Vmax. (Vmax-200 mmol/s)
Assume that a solute has a D* of 2.03 x 10-9 m2 / Sec and that w is equal to 0.5. If landfill leachate with a chloride concentration is next to a clay liner that is 2 m thick and the concentration of chloride is 2315 mg/L, what would be the chloride concentration at the outside of the liner after 25 years of diffusion. ? ignore any effects of advection. Defining a "release" from the landfill as a concentration exceeding 1 mg/l, how long will it take for a release to occur by diffusion alone? Provide answer in years to the nearest tenth. Eg: 10.3