Hemoglobin A has a pI value of 5.6 whereas the variant hemoglobin M has an aspartate residue in place of the normal valine at position 85 of the alpha chain. What effect will this substitution have on the electrophoretic behavior of the protein at pH 7.0. (explain clearly the reason for your answer)

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Q: Explain the difference in the pKa values of the carboxyl groups of alanine, serine, and cysteine.

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Q: For lysine and arginine, the isoelectric point, pI, occurs at a pH where the net charge on the…

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Q: Calculate the pH at which the e-amino group of lysine is 25% dissociated.

A: Step : 1             Calculate the pH at which the e-amino group of lysine is 25% dissociated.

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Q: What trend can you suggest for the stability of proteins under varying pH

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Q: Draw the tripeptide Alanine-Cysteine-Serine (R1 = CH3, R2 = CH2SH, R3 = CH2OH) under the following…

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Q: Below which is false about determining protein three-dimensional structure?

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Spectroanalytical Methods

Spectroanalytical methods measure the wavelength and intensity of radiations that are either emitted or absorbed by a molecular or atomic species. These methods are useful for evaluating the electronic or atomic arrangement of the molecules of a given analyte.