Q: What types of samples can be measured with Circular Dichroism? Can information be extracted?
A: Circulate dichroism is the difference in the absorption of left handed circularly polarised…
Q: What is the function of the mordant used in the Gram staining procedure? If the iodine step is…
A: Gram stain or Gram staining, likewise called Gram's strategy, is a technique for staining used to…
Q: n in detail gram stain biochemistry, procedures, gram-positive and gram negative cells.
A: Gram stain, a wide used microbiological, biochemical staining technique that greatly aids in the…
Q: What is the purpose of the pour plate technique? If a pure culture is used to inoculate the plate,…
A: PURPOSE OF POUR PLATE TECHNIQUE The pour plate techniques can be used to determine the number of…
Q: Why is dilution a necessary part of pure culture preparation?
A: The pure culture consists of a single microorganism. This culture helps to easily evaluate the…
Q: How does pasteurization affect the number of bacteria present in milk? What differences, if any,…
A: To control microbial growth or contamination there are a number of aseptic techniques developed that…
Q: hat is the advantage of culture-independent analysis over culture-dependent analysis of…
A: An unknown microorganism can be identified and classified in a variety of ways. They are divided…
Q: In the preparation of a bacterial smear, why is there a need to fix the bacteria to the slide? Aside…
A: Several laboratory techniques necessitate smear preparation. A smear can be made using either broth…
Q: Give two reasons for heating the slide after the smear is air dried?
A: Smear is the thin film of the sample that is spread over the slide to be observed in the microscope.…
Q: What is the difference between culture-based technique and culture-independent techniques based on…
A: A microbiological culture, also known as a microbial culture, is a way of multiplying microbial…
Q: What are the disadvantages of doing a total microbial count by examining wet mounts under the…
A: Ans- Wet mounts have the following drawbacks: The benefit of being able to observe movement can also…
Q: What are the pros and cons of using the TCE stain-free method versus a traditional gel staining…
A: PAGE (polyacrylamide gel electrophoresis) is a well-known method for separating, detecting, and…
Q: In gram staining, could colors other than red be used as a counterstain?
A: A counterstain is a stain with color contrasting to the principle stain, making the stained…
Q: Why is it sufficient to stain cells from a SDA (Sabouraud Dextrose Agar) plate with crystal violet…
A: Introduction : Sabouraud agar, often known as Sabouraud dextrose agar (SDA), is a peptone-containing…
Q: What is a pure culture?
A: Introduction Culture is referred to th process of growing the cell outside the animal or plant body…
Q: Is the flagellar stain a Positive or Negative Simple Stain protocol?
A: Staining is a technique that is used to enhance contrast in samples, generally at the microscopic…
Q: What are pure cultures and why are they important? How are spread plates, streak plates, and pour…
A: In science and related fields, a culture plate is a low level lined lab holder for growing a layer…
Q: Why is heat-fixed procedure in bacterial smear preparation not ideal for capsular staining?
A: In microscopic studies, the specimen needs to be prepared for an optimized vision under a…
Q: Why should culture media after inoculation be incubated at an optimal temperature immediately?
A: Culture media, also called as growth media, are particular combinations of nutrients and other…
Q: Why is PBS buffer preferred when preparing a monolayer cell culture?
A: PBS (phosphate buffer saline) is a buffer solution which is a water based salt solution containing…
Q: Why is mordant necessary for the staining of bacterial flagella? Answer in not more than 3…
A: In contrary to negative staining, which leaves the bacteria unstained against a dark backdrop,…
Q: Why is it desirable that most cultures be inspected after 15 to 18 hours of incubation?
A: Introduction Microbial culture is a microbiological technique that involves actively multiplying…
Q: a. Why must heat be used with the application of the primary stain during endospore staining?
A: Defination- An endospore is a non -reproductive structure which is dormant and tough produced by…
Q: What is the secondary stain in both acid-fast stain methods?
A: The bacteria is the unicellular prokaryotic organism that maintains a definite shape since it…
Q: Which step of the gram stain procedure is most critical?why?
A: Gram staining is one of the most common laboratories test to identify the different bacterial…
Q: When using the blue/ white colony screening method, what do blue colonies represent ?
A: Blue- white screening is used for the identification of recombinant bacteria.
Q: Of what practical importance are air borne microorganisms to the laboratory workers? What…
A: Questions related to the microbiology labs. Note: each questions are answered under particular…
Q: What is selective medium?
A: Description of a selective medium.
Q: What should be considered when preparing a monolayer cell culture?
A: Monolayer culture, also known as adherent culture, is a form of animal cell culture in which cells…
Q: is staphylococcus aureus cells under 40 times magnification bigger than onion cell or cheek cell?
A: Staphylococcus aureus is a Gram-positive, round-shaped bacterium that belongs to the Firmicutes and…
Q: How can moist heat sterilization can sterilize antibiotic solution?
A: Moist heat sterilization is a technique that uses heated, increased pressure steam to sterilize an…
Q: What is the procedure for methylene blue stain on e.coli?
A: Methylene Blue is a cationic stain, which binds to negatively charged parts of the cells, such as…
Q: Which step in the Gram stain procedure is most prone to error? If done incorrectly, how might that…
A: The differential stain is used to distinguish between different structures and cellular components.…
Q: How might a synthetic detergent be superior to a normal soap?
A: INTRODUCTION - Soaps are sodium or potassium fatty acid salts made from hydrolysis of fats in…
Q: What other fields of science besides microbiology is the aseptic technique used?
A: Aseptic technique refers to the procedures that prevent the contamination of microorganisms. They…
Q: How does the agar dilution method differ from streaking toobtain isolated colonies?
A: Both these are methods to culture microorganisms and have their respective pro and cons as mentioned…
Q: what is the function of each one of the Gram-stain reagents?
A: Gram staining is the technique used to classify the bacterial species into broad groups based on the…
Q: Why do we need to stain microorganisms?
A: Microbiology is the study of microbes. Microorganisms include bacteria, fungus, archaea, and…
Q: What is the function of a mordant, and which reagent serves this purpose in the Gram stain…
A: An extensively used microbiological method of staining is called a Gram stain. It significantly…
Q: How does the age of a culture affect the Gram stain reaction? What is an optimum culture age for a…
A: To identify the unknown bacteria this gram staining is used. This staining technique differentiate…
Q: what is the procedure of solving the probelems of cultures those are suffering severely due to…
A: This is the defense compound of the plant and are toxic in nature because of that preventing plant…
Q: bacteria require staining before microscopic examination?
A: Bacteria are single-celled organisms with a small size. Bacteria can be found practically everywhere…
Q: How does smear preparation of cells from a liquid medium differ from preparation of cells from a…
A: Answer: Smear Preparation is the method in microbiology laboratories to place the small amount of…
Q: What is diluent? Why is it useful for working with bacterial samples?
A: Dilution is done to decrease the concentration of the original sample that is being tested. Dilution…
Q: What color wouod gram negative cells appear if the decolorizing step was left out of staining…
A: Gram staining method: This technique is useful in identifying two groups of bacteria based on the…
Q: Briefly, in your own words, describe the steps of the gram staining procedure.
A: The bacterial kingdom is classified into Gram positive and Gram negative bacteria depending on their…
Q: What are the principle and basic concepts of GRAM STAIN?
A: Gram staining is among the most important microbiology staining procedures. It was first invented in…
Q: Why should agar media be completely dissolved before they are dispensed in tubes and plates? What…
A: Introduction A growth medium, also known as a culture medium, is a solid, liquid, or semi-solid…
Q: What staining technique is used here? What is the primary stain, decolorizing agent, and counter…
A: Gram Staining is the common, important, and most used differential staining techniques in…
How does smear preparation of cells from a liquid medium differ from preparation of cells from a solid medium?
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- What is the purpose of the pour plate technique? If a pure culture is used to inoculate the plate, why are some colonies bigger than others?how can ultraviolet be used to control microbial growth. which specific UV can be used to control microbe?Why is PBS buffer preferred when preparing a monolayer cell culture?
- How does smear preparation of cells from a liquid medium differ from preparation of cells from a solid medium? Mark all that apply: A smear made from liquid media will be thinner than a smear made from solid media A smear made from solid media doesn't have to be fixed but a smear from liquid media does A smear made from liquid media will be more thick than a smear made from solid Water must be added to a smear made from solid media but not liquid media A smear made from liquid media doesn't have to be fixed but a smear from solid media does Water must be added to a smear from liquid media but not from solidWhat is selective medium?Is heat fixation used on bacterial smears that will undergo a capsule stain or flagella stain procedure? Why or why not?