Three grams of soil from the ground were added to 27 mL of sterile water and shaken vigorously. After the soil particles settled, 0.1 mL of this was added to 9.9 mL of sterile water. This was further diluted by 4 successive 1/10 dilutions. One mL from the last dilution was used to prepare a pour plate. After incubation, 289 colonies were present on this plate. What was the number of colony-forming units/gram of the soil?

Three grams of soil from the ground were added to 27 mL of sterile water and shaken vigorously. After the soil particles settled, 0.1 mL of this was added to 9.9 mL of sterile water. This was further diluted by 4 successive 1/10 dilutions. One mL from the last dilution was used to prepare a pour plate. After incubation, 289 colonies were present on this plate. What was the number of colony-forming units/gram of the soil?

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter6: Basic Clinical Chemistry

Section6.2: Chemistry Specimen Collection And Processing

Problem 10RQ

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