The best fit curve line to an absorbance vs. concentration plot for standard solutions of a dye has a slope of 0.1815 mL/mg and intercept of 0.0477. A sample solution prepared of the same dye has an absorbance of 0.464 at the wavelength used to establish the Beer's law plot. What is the concentration of dye in the prepared solution? Select one: O a. 0.132 mg/mL b. 2.29 mg/mL O c. 0.464 mg/mL O d. 2.82 mg/mL e. 0.0365 mg/mL Next page
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- Determine the concentration of your prepared Iron (III) Salicylate Dianion solution (pure ASA) in the 10.0 mL of 1.0 M NaOH in mg/mL (Part D) using Beer's Law plot. Hint:You need to use the measured absorbance reading of your prepared Iron (III) dianion (absorbance reading: 0.83). Information from the plot: Standard: Concentration ofASA in Standards (mg/mL) A: 0.18 B: 0.14 C: 0.10 D: 0.070 E: 0.040 Mass of salicylic acid (g) 0.17g Volume of acetic anhydride (mL) 0.34mL Mass of filter paper (g) 0.35gMass of "impure" ASA and filter paper (g) 0.49g Mass of prepared "impure" ASA (g) 0.14g Melting point range of prepared ASA (°C) 20.9 °C Melting point range of standardASA (°C) 18.5°C Percent purity: 28.57% Percentage yield: 66.7% Chemical equation: C7H6O3 + C4H6O3pure ASA knowing that two consecutive dilutions were done to prepare the solution. Show all of your work.C9H8O4 + C2H4O2 b) Then, calculate the mass (in mg) ofComplete the information on these tables: wavelength 470 nm conc Um absorbance % transmission 700 0.45 35.58% 500 0.32 47.78% 300 0.19 64.2% 100 0.06 86.23% wavelength 502 nm conc Um absorbance % transmission 700 1.08 8.37% 500 0.77 16.99% 300 0.46 34.57% 100 0.15 70.15% Which mathematical relationship is most useful to relate concentration to the amount of light passing through the solution? (% Transmission, or Absorbance), Why? How does the slope of the absorbance/concentration graph vary with wavelength? Is there an “optimum” wavelength for your KMnO4 sample?The absorbance values at 250nm of 5 standard solutions, and sample solution of a drug are given below: Conc. (ug/ml) A 250 nm10 0.16820. 0.32930 0.50840. 0.66050 0.846Sample. 0.661Calculate the concentration of the sample
- a) Determine the concentration of your prepared Iron (III) Salicylate Dianion solution (pure ASA) in the 10.0. mL of 1.0 M NaOH in mg/mL (Part D) using the Beer's Law plot completed in Question 1. Hint: You need to use the measured absorbance reading of your prepared Iron (III) dianion. b) Then, calculate the mass (in mg ) of pure ASA knowing that two consecutive dilutions were done to prepare the solution. Show all of your work. given information: beer's law Standard: Concentration of ASA in Standards (mg/mL) A: 0.18 B: 0.14 C: 0.10 D: 0.070 E: 0.040 Sample: Absorbance reading standard A: 1.25 standard B: 0.96 standard C: 0.74 standard D: 0.50 standard E: 0.23 the absorbance reading for my prepared iron (III) dianion is 0.83 the volume is 25.0mL b is the path length of the cuvette (assume 1 cm) the molecular weight of Iron (III) Salicylate Dianion is 330.09g/mol (correct me if i am wrong) i do not have the molar absorptivity, but the equation is 7,1*x +0.017 for the slope-intercept…A standard curve for glucose analysis was prepared.The slope (m) was found to be 1.341 while the y-intercept (b) was -0.333. Calculate the concentration (x) of glucose having an absorbance (y) of 0.151. 0.067 0.361 0.858 0.136Suppose that a solution has an absorbance of 0.250 at a wavelength of 450 nm. If the concentration of the solution is 22 μM, what is the value of the molar absorptivity? The data were taken with a standard 1-cm cuvette. molar absorptivity in (L cm^−1 mol^-1):
- An unknown concentration of [FeSal]+ gave an absorbance reading of 0.301. Calculate the concentration of the solution, given that the slope of the standard curve was 1280 and the y intercept was -0.00821.When using cuvettes to measure the absorbance of a solution, why is it necessary to only hold the cuvettes near the top?The nickel level in a soil sample was determined using AAS. A 3.0 g sample was dissolved in acid and then diluted to a total volume of 100.0 mL. The absorbance of this solution determined at a wavelength of 232 nm, was found to be0.3. Several standard solutions were tested under the same conditions and a calibration curve shown below was obtained. Calculate the mass of nickel in mg.
- Calculate the molar absorptivity of a solute when its measured absorbance is 1.050 with a concentration of 1.45 x 10-3 M. Assume the path length of the cell is 1.0 cm.In determining the amount of copper and the identity of the copper compound in the unknown sample, a 100-mL solution containing 120.8 mg of the sample was initially prepared. Then, 5.00 mL of this solution was pipetted and diluted with deionized water in another 100-mL volumetric flask. The absorbance of this diluted solution was determined three times at the analytical wavelength. The concentration of Cu is 12.678 mg/L, what is the mass of Cu in the analyzed solution and the mass of Cu in the unknown sample?Marco diluted a stock solution of Yellow #5 dye and obtained the equation y=13.09x after plotting his Beer's Law data. Using the equation Marco got, determine the concentration, in M, of Yellow #5 in a solution with an absorbance of 0.436.