In normal mitochondria, which of the following is true for the rate of NADH consumption (oxidation)? Select all that apply Be very low if the ATP synthase is inhibited, but increase when an uncoupler (equalizer of H+ concentration) is added decrease if mitochondrial ADP is depleted Increase in active muscle and decrease in inactive muscle decrease when cyanide is added to prevent electron transfer through the electron transport chain
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- The Relative Efficiency of ATP Synthesis in Noncyclic versus Cyclic Photophosphorylation If noncyclic photosynthetic electron transport leads to the translocation of 7 H+/2e- and cyclic photosynthetic electron transport leads to the translocation of 2 H+/e-, what is the relative photosynthetic efficiency of ATP synthesis (expressed as the number of photons absorbed per ATP synthesized) for noncyclic versus cyclic photophosphorylation? (Assume that the CF1CF0-ATP synthase yields 3 ATP/14 H+.)When rotenone is added to actively respiring mitochondria,the ratio of NADH to NAD+ increases, but the FADH-/FADratio remains unchanged. What step in the system is beinginhibited?If decanoic acid (10 : 0) undergoes complete β-oxidation in mitochondria, how many net ATP is yielded? (NADH = 2.5 ATP; FADH2 = 1.5 ATP; Acetyl CoA = 10 ATP) Answer in number only
- which of the following woudl decrease the number of atp molecules generated per nadh molecule in the electron transport chain? a. increasing the energy of the electrons nadh transfer to complex I b. reducing the number of protons required by ATP synthase to produce an ATP moelcule c. having nadh transfer its elections to complex III instead of complex I d. increasing the amount of oxygen available to the cellPer NADH consumed by Complex I, indicate the number for each, AS those reducing equivalents flow through the electron transport chain. In other words, if "XYZ" molecule gets reduced at one step and then re-oxidized at the next step, that would count as 1 XYZ reduced. Think Total not Net. a)Total # of Cytochrome C proteins reduced b)Total # of H+ pumped across membrane c)Total # of H2O producedWhich of the following conditions must be met within the cell In order for the enzyme pyruvate carboxylase to be activated? Select all that apply. NADP+ levels must be high Phosphofructokinase must be in the activated R-state Beta-oxidation of fatty acids happening in the mitochondrial matrix AMP levels must be high ATP levels must be high
- Complex II of electron transport chain is involved in the KREB'S Cycle during the conversion of succinate to fumarate?true or false all enzymes of the citric acid cycle are located in the cytoplasm except succinate dehydrogenase which is bound to the inner mitochondrial membrne. true of false glucagon is a hormone that activates glycogenolysis-true or false high concentration of NADPH increases the rate of the pentose phosphate pathway by stimulating glucose 6-phosphate dehydrogenase- true or falseConsider a 24:1 △cis-9 fatty acid in the mitochondrion. For each fatty acid given, determine the following. Gross ATP from b-oxidation cycles Gross ATP from acetyl CoA produced Gross ATP from conversion of propionyl CoA (if applicable) Total number of ATP deducted Total net ATPH+ flow down their gradient from the intermembrane space into the mitochondrial matrix through _____________, the smallest known rotary motor, to make _____________. the electron transport chain; ATP ATP synthase; ATP the electron transport chain; NADH ATP synthase; O2
- Assuming all the NADH+H+ and FADH2 are used for oxidative phosphorylation, how many net ATP are made from 1 molecule of glucose? (hint: don’t forget to also include substrate level phosphorylation)When the antibiotic X is added to actively respiring mitochondria, several things happen: the yield of ATP decreases, the rate of O2 consumption increases, heat is released, and the pH gradient across the inner mitochondrial membrane increases. Does X act as an uncoupler or an inhibitor of oxidative phosphorylation? Explain the experimental observations in terms of the antibiotic’s ability to transfer K+ ions across the inner mitochondrial membrane.which statement is false? a. Electrons do not usually flow through the electron-transport chain to oxygen unless ADP is simultaneously phosphorylated to ATP. b. Oxidative phosphorylation requires a supply of NADH or other source of electrons at high potential, oxygen, and ADP. c. The rate of oxygen consumption by mitochondria increases markedly when ADP is added and then returns to its initial value when the added ADP has been converted into ATP. d. The most important factor in determining the rate of oxidative phosphorylation is the level of ADP and inorganic phosphate.