In test tube 1 (no puromycin) you get a polypeptide that is 90 amino acids long. At least how many bases was the coding region of the mRNA that you added? O 30 bases O 90 bases 900 bases 273 bases

Human Heredity: Principles and Issues (MindTap Course List)
11th Edition
ISBN:9781305251052
Author:Michael Cummings
Publisher:Michael Cummings
Chapter9: Gene Expression And Gene Regulation
Section: Chapter Questions
Problem 10QP: The pre-mRNA transcript and protein made by several mutant genes were examined. The results are...
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You perform a translation experiment with mRNA and all required molecules in two
identical test tubes. You add limiting amounts of the antibiotic puromycin to test
tube 2 only.
Puromycin is a molecule that has structural similarities to the 3' end of a charged
TRNA. It can enter the ribosome and be incorporated into the growing protein. When
puromycin is incorporated into the polypeptide, it stalls the ribosome and the
polypeptide is released.
You do not know if puromycin recognizes a specific codon or not.
You examine the length of the polypeptide produced in both test tubes.
In test tube 1 (no puromycin) you get a polypeptide that is 90 amino acids long.
At least how many bases was the coding region of the mRNA that you added?
30 bases
90 bases
O 900 bases
O 273 bases
Transcribed Image Text:You perform a translation experiment with mRNA and all required molecules in two identical test tubes. You add limiting amounts of the antibiotic puromycin to test tube 2 only. Puromycin is a molecule that has structural similarities to the 3' end of a charged TRNA. It can enter the ribosome and be incorporated into the growing protein. When puromycin is incorporated into the polypeptide, it stalls the ribosome and the polypeptide is released. You do not know if puromycin recognizes a specific codon or not. You examine the length of the polypeptide produced in both test tubes. In test tube 1 (no puromycin) you get a polypeptide that is 90 amino acids long. At least how many bases was the coding region of the mRNA that you added? 30 bases 90 bases O 900 bases O 273 bases
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