Large proteins travel/migrate fast
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- There can be more than one correct answer Which of the following methods can seperate proteins based on their mass? a.) centrifugation b.) gel filtration chromatography c.) SDS polyacrylamide gel electrophoresis d.) ion exhange chromatographyPlease pick among these 4 Protein chromatography is typically column-based:— lon-exchange— Reverse-phase/Hydrophobic interaction— Gel-filtration— AffinityTotal Protein Determination Spectroscopy Values CREATE CALIBRATION CURVE? DRAWN CONCENTRATION AND ABSORPTION CURVE
- option isoelectric focusinggel filtrationsalting outdifferential centrifugationEdman degradationion exchangedialysisLabel the void volume (V0), elution volume (Ve) and total elution volume (Vt) appropriately on the graph for the gel filtration chromatography using a column experiment.You make 100 ml of a stock solution for mounting sections, that contains 0.5g gelatin. You take 10 ml of this stock solution and add it to 990 ml 0.1 X phosphate buffered saline. The concentration of gelatin in this diluted solution is: 0.5% 0.05% 0.005% 0.0005% 0.00005%
- With the aid of diagrams, explain the processes and principles of each of the following methods: (a) Gel filtration chromatography on Sephadex G50 b.SDS-PAGEDNA concentration is 3.75 ng/ ul = the dilution factor is 1:______ we add____ uL dna extract to ____ ul buffer final dilution concentration _____ ng/ ul please explainHow is it that smaller molecules move through a gel-filtration column more slowly than larger molecules, whereas in SDS polyacrylamide-gel electrophoresis (SDS-PAGE) the opposite is true: larger molecules move more slowly than small molecules?
- Briefly explain the principle behind the following techniques:(a) ion-exchange chromatography (b) gel filtration chromatography (c) affinity chromatographyIn a polyacrylamide gel electrophoresis, the gel consists of specific gel components. name themDILUTION COLONY COUNT CFU/mL 1:106 155000 1:107 15500 1:108 1550 1:109 155 Given these values how would I fill in the rest of this serial dilution table? Also, what would be a 1:1 CFU/mL value based on this table?