Method DNAS sensitivity to DNase I was tested on different tissues and at different times in development. Key DNA highly sensitive to DNase I Embryonic globin gene globin genes Adult Chicken DNA U aD Results Erythroblasts first 24 hours Before hemoglobin synthesis, none of the globin genes are sensitive to DNase I digestion. a4 After globin synthesis has begun, all genes are sensitive to DNase I, but the embryonic globin gene Uis the most sensitive. Erythroblasts 5 days In the 14-day-old embryo, when only adult hemoglobin is expressed, adult genes are most sensitive and the embryonic gene is insensitive. Erythroblasts 14 days Globin genes in the brain- which does not produce globin-remain insensitive throughout development. Brain cells throughout development Conclusion: Sensitivity of DNA to digestion by DNase I is correlated with gene expression, suggesting that chromatin structure changes in the course of transcription. DNase I sensitivity is correlated with the transcription of globin genes in chick embryos.
Method DNAS sensitivity to DNase I was tested on different tissues and at different times in development. Key DNA highly sensitive to DNase I Embryonic globin gene globin genes Adult Chicken DNA U aD Results Erythroblasts first 24 hours Before hemoglobin synthesis, none of the globin genes are sensitive to DNase I digestion. a4 After globin synthesis has begun, all genes are sensitive to DNase I, but the embryonic globin gene Uis the most sensitive. Erythroblasts 5 days In the 14-day-old embryo, when only adult hemoglobin is expressed, adult genes are most sensitive and the embryonic gene is insensitive. Erythroblasts 14 days Globin genes in the brain- which does not produce globin-remain insensitive throughout development. Brain cells throughout development Conclusion: Sensitivity of DNA to digestion by DNase I is correlated with gene expression, suggesting that chromatin structure changes in the course of transcription. DNase I sensitivity is correlated with the transcription of globin genes in chick embryos.
Biochemistry
6th Edition
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Reginald H. Garrett, Charles M. Grisham
Chapter30: Protein Synthesis
Section: Chapter Questions
Problem 1P
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Question
Based on the sensitivity of DNA to DNase I, as illustrated in Figure , which type of chicken hemoglobin (embryonic or adult) is likely to be produced in the highest quantity in the following tissues and developmental stages?
Q. Erythroblasts at day 5
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