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- In reversed phase partition chromatography Select one: a. the least polar compound elutes first b. the smallest molecular weight compound elutes first c. the most polar compound elutes first d. the largest molecular weight compound elutes first e. the lowest boiling compound elutes first f. the highest boiling compound elutes firstSeperation of Amino Acids by thin layer chromotography Lab. Please answer #12 and #13 as I am very confused. Thank you for your help!Calculate the amount of phycocyanin in Sample 1 in mg where A620 = 0.193 and A650 = 0.095, taking into account the dilution factor of 100 ul, and the total volume of extract 45ml. Note your answer to 2 decimal places.
- Which is more polar, the water solvent or the chromatogrphy paper?Why do the ‘% Area’ values on the HPLC not correspond directly to composition by mole?Explain briefly the differences in Rf values of the pigments that you have separated from spinach juice. What properties must the pigment have to travel further in the chromatogram? Consider the structures of cholesterol and ?-carotene. Which do you think will have the highest Rf value if the solvent used was acetone - hexane (20:80)? Why?
- You performed an isocratic reversed-phase HPLC separation of phenol, toluene and catechol using a DAD detector set at 275nm and obtained the chromatogram shown below. What are the polarities of the mobile phase and stationary phase in this separation? What order would you expect these compounds to elute in? Why? What changes could you make to the mobile phase to decrease the elution time of the last compound and improve its peak shape? If all three species are present in the analyzed solution at the same concentration, what might account for different in peak height for the first two compounds eluting from the column? What change to the instrument set-up could you make to increase the detector response of this analyte?Calculate the amount of phycocyanin in Sample 1 in mg where A620=0.211 and A650=0.086, taking into account the dilution factor as per question 6 (100ul), and the total volume of extract as per question 4 (140ml) . Note your answer to 2 decimal placesIn HPLC, why is purging being done before analysis of samples. Outline the procedure on how purging is performed.
- which subsatomic particles contriubte to the chrage of Cu2+, which do notwhy are these sterically hindered what does it even mean to be stericaly hinderedCompute for the appropriate amount (mL_) of the compositions of a 10 mL 2x CTAB buffer for DNA extraction. a. 2% w/v CTAB b. 1.4 M sodium chloride c. 100 mM Tris-Cl d. 20 mM EDTA e. 2% polyvinylpyrrolidone MW 40 000