Sugar Molisch's Solution Test Glucose Sucrose Lactose Hydrolysis: Samples Sucrose hydrolysate Starch hydrolysate Moore's Test COLOR OBSERVED Benedict's Fehling's Test Test Result Seliwanoff's Test
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- Given Tagatose, Briefly explain its expected reaction (based on their structural formula) to the different qualitative tests for carbohydrates which are: (a) Molisch’s test (b)Fehling’s test (c) Bial’s testDIFFERENCE AMYLOSE AMYLOPECTIN Structure Mol Size Degree of branching IODINE TEST INDICATION Solubility in H20 RATIOGiven Ribose, Briefly explain its expected reaction (based on their structural formula) to the different qualitative tests for carbohydrates which are: (a) Molisch’s test (b)Fehling’s test (c) Bial’s test
- Choline test procedure (chemical reagents added), positive results/observations, and positive for (provide details like functional group responsible and list of lipids that will yield positive results)Given Sorbitol, Briefly explain its expected reaction (based on their structural formula) to the different qualitative tests for carbohydrates which are: (a) Molisch’s test (b)Fehling’s test (c) Bial’s testGiven Tagatose, Briefly explain its expected reaction (based on their structural formula) to the different qualitative tests for carbohydrates which are: (a)Barfoed’s test (b)Seliwanoff’s test (c) Iodine’s test
- Given Raffinose, Briefly explain its expected reaction (based on their structural formula) to the different qualitative tests for carbohydrates which are: (a) Molisch’s test (b)Fehling’s test (c) Bial’s testReagents: 8 ml conc. Sulfuric acid, 1.5 ml alpha naphthol, 1 ml iodine in KI, 4 ml conc. HNO3, 4 ml Seliwanoff’s reagent, 3 ml Bial’s-orcinol reagent, SAMPLE: 5% of lactose, sucrose, glucose, galactose, fructose, maltose, starch and xylose ( 10 ml each) Materials: 12 test tubes 20 ml, test tube rack, test tube brush, test tube holder, alcohol lamp, tripod, wire gauze, water bath, (10 ml) graduated cylinder, dropper, stirring rod, 3 droppers, 250 ml beaker Procedure: 1. Molisch Test – (use all 8 samples) Place 1 ml of each sample in different test tubes. Add 1 drops of alpha napthol reagent in each tube. Mix thoroughly. Incline the tube and CAREFULLY allow 1 ml of conc. H2SO4 to flow down the side of the tube. Note the color formed at the zone of contact between 2 liquids. 2. Iodine Test – ( use all 8 samples) place 1 ml of the sample in a test tube. Add a drop of iodine. Observed color produced. Heat (direct heating) and note change in color. Cool and record again the result.…Identify what test is being described: Test for the presence of indole group ? Hydrolysis Denaturation Heat denaturation Organic solvent denaturation Biuret test Hopkins – Cole Reaction Millon’s test Ninhydrin Test Sulfur test Xanthroproteic Test Chromatography Paper chromatography Competitive inhibition Noncompetitive inhibition Rancidity Hydrogenation Identify what test is being described: Process of adding hydrogen to unsaturated fatty acids ? Hydrolysis Denaturation Heat denaturation Organic solvent denaturation Biuret test Hopkins – Cole Reaction Millon’s test Ninhydrin Test Sulfur test Xanthroproteic Test Chromatography Paper chromatography Competitive inhibition Noncompetitive inhibition Rancidity Hydrogenation Identify what test is being described: Yields blue color solution for proteins containing fewer than two peptide bonds. ? Hydrolysis Denaturation Heat denaturation Organic solvent denaturation Biuret test Hopkins – Cole Reaction Millon’s test Ninhydrin Test Sulfur test…
- Tell me about: Qualitative Analysis of Carbohydrates. Thank you in advance.Based on this video https://www.youtube.com/watch?v=rKng5-ij6kQ Provide a schematic diagram for the Barfoed’s test methodologies in determining the presence of carbohydrates. Also, give the basic principle for the test. (not a graded question, video is very brief)BARFOED’S TEST FOR MONOSACCHARIDESPlace 1 mL (20 drops) of each 1% carbohydrates solution in separate test tubes. Prepare a control with 1 mL water. Add 4 mL Barfoed’s reagent. Place the test tubes in boiling water bath for 10 minutes. Remove the test tubes and cool in cold water. Record your observations. A precipitate of cuprous oxide which may be green to red, indicates that a monosaccharide is present. Record your result and conclusion. Identify the compounds that are monosaccharides. SELIWANOFF’S TEST FOR KETOHEXOSESPlace 5 drops of 2% carbohydrate solution in separate test tubes. Prepare a control with 5 drops water. Add 1 mL of Seliwanoff’s reagent to each test tubes and heat. Record your results. The rapid formation of a bright red indicates that a ketohexose is present. Identify the compounds that are ketohexoses. IODINE TESTS FOR POLYSACCHARIDESPlace 2 mL of each carbohydrate solution in separate test tubes. Prepare a control with 2 mL of water. Add 1 drop of iodine solution…