3 Two dialysis membranes each containing a methylene blue solution were placed in beakers of water and theirelution was observed for 60 minutes. At 5, 15, 45, and 60 minutes, the absorbance of the water wasdetermined using a colorimeter. The absorbance vs. time plot for each membrane is given below. Whichdialysis membrane has a higher pore cutoff value? Why?0,16100Membrane 1900Membrane 21002001000ot(una) aua1

In dialysis, colloids are separated from dissolved ions or molecules of small dimensions or…

Answered: sunganegy Two dialysis membranes each…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Similar questions

Which of the following techniques can be applied to fractionate intracellular compartments? a. Subcellular fractionation & density gradient equilibrium centrifugation b. Density gradient equilibrium centrifugation & ion exchange chromatography c. Subcellular fractionation, density gradient equilibrium centrifugation & ion exchange chromatography d. Subcellular fractionation & ion exchange chromatography
You set up the following two tubes: Tube 1: 1mL of alkaline phosphatase + 4mL of 400 µM p-NPP Tube 2: 1mL of alkaline phosphatase + 4mL of 200 µM p-NPP Which tube would have a faster reaction rate? Explain your reasoning (why does concentration of p-NPP matter? Relate it back to week 4 lab concepts). Week 4 lab concepts: - concentration gradient - differentially permeable membrane - solute, solvent, diffusion, osmosis, hypotonic, isotonic, hypertonic, molarity, osmolarity
Table 2. Volume of BSA, protein content, and absorbance readings of reference solutions Solution Volume of BSA standard solution (μL) Protein content(μg/mL) Absorbance value At 595 nm 1 0 0 0 2 10 1 0.022 3 30 3 0.065 4 50 5 0.106 5 70 7 0.178 6 100 10 0.299 7 120 12 0.380 Make a graph by plotting the absorbance values versus the BSA protein content (in μg) for theseven reference solutions. When constructing the graph, be…
Solution A contains 100 mmol/L glucose and Solution B contains 50 mmol/L NaCl. Assume that gNaCl is 2.0, σglucose is 0.5, and σNaCl is 0.8. If a semipermeable membrane separates the two solutions, what is the direction of water flow across the membrane?
With regard to the tubes containing sucrose, how would you classify the situation in terms of solvent movement ? ( isotonic, hypotonic, or hypertonic)? Explain.
Define the following terms: a. solvation sphere b. amphipathic c. micelle d. hydrophobic effect e. clathrate
You have two types of cells (animal cells and plant cells) that you will place in a variety of conditions. Please describe what would happen to each of those two cells in the following solutions, i) isotonic solution; ii) hypertonic solution; iii) hypotonic solution.
With the principles of GFC, which of the following statements is incorrect?a. For small molecules, their elution volume, Ve > VOb. For a very large molecule, its elution volume, Ve = Voc. Blue dextran is used to determine the void volume because it is too large and cannot get into the beads and therefore excluded from the gel.d. Low molecular weight molecules are freely accessible to the gel particles, and elute at a volume equal to the internal pore volume.In an SDS-PAGE experiment, protein X has an Rf = 0.75, while protein Y has an Rf = 0.30. Which of the following statements are FALSE during the electrophoretic run?I. X has a greater charge-to-mass ratio than Y, since X has a smaller MW.II. X has a lower MW, and thus experiences less friction with the gel matrix.III. X has more hydrophobic groups, thus is in a more linearized state.IV. X is globular, while protein Y is rod-like, thus X has higher mobility.V. X is affected by less magnitude of electric field because of smaller…
Depicted is a rod-shaped bacterium that can be approximated as a cylinder with two spherical caps, r = 0.5 micron. If the difference between the osmolarities inside and outside is 10 mosM, what would be the tension in the bacterial envelope? Is it uniform? (concentrations are expressed in mol/L)
“The processes of life require a wide variety of ions and molecules tobe close to each other and be soluble in a common medium”. discuss the importance and application ofthis statement in linking the unique properties of water (as universalintracellular and extracellular medium) to the dissolution and absorption ofdrugs in the body.
What is isotonic solutions hypothetical concentration of solvent and solute?
Your team suggests changing the temperature of the oil spill site in order to enhance the bioremediation process. Do you consider this decision effective? Why and why not? Explain, in your answer, the effect of changing the zone tension on the homeostasis capacity of the bacteria you use and their ability to withstand the osmotic pressure of the new surrounding solvent. In your answer, state how osmosis works and state the degree of tension of the solution (isotonic, hypertonic or hypotonic). Explain why osmosis is really just a special case of facilitated diffusion

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