Suppose you want to figure out what genes are involved in the production of proline (Pro) in a certain bacteria. (1) How would you go about designing a mutant screen to do so? (2) Suppose your screen identified 5 mutants that barely grew without being given Pro in the medium. How would you go about determining the number of different genes these mutants represent?

Suppose you want to figure out what genes are involved in the production of proline (Pro) in a certain bacteria. (1) How would you go about designing a mutant screen to do so? (2) Suppose your screen identified 5 mutants that barely grew without being given Pro in the medium. How would you go about determining the number of different genes these mutants represent?

Biology Today and Tomorrow without Physiology (MindTap Course List)

5th Edition

ISBN:9781305117396

Author:Cecie Starr, Christine Evers, Lisa Starr

Publisher:Cecie Starr, Christine Evers, Lisa Starr

Chapter10: Biotechnology

Section: Chapter Questions

Problem 1CT

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In this problem, just put the order of the intermediates on the pathway, starting with P and ending with Z and explain your reasoning. (So to get you started, notice the class 1 mutants—and there is only mutant in this class, which is mutant #5—won’t grow on minimal medium, but will grow if you give it substance Z. However, giving it substances, p, w, x, or y won’t help. This means that the gene that is mutated lies on the pathway in a place that the Z substances is to its right and all the other substances are to its left. The easiest next one to look at is the line with 2 pluses, and then the line with 3 pluses, and then the line with 4 pluses).
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