The at toin gere was souted from the bacterium Bacilus thuringiersis Chromosome Bacilius thuringiensis Bt toxin gene ard cloned vto E col. E coli plasmid The full-ength t gene and neo gene Bt gene ragments of the gene were gated to the ne gene -Ful-length Bt gene neo Cene Partial Bt genes constructs nee Promoter Restriction site PolyA) mee consensus sequence Expression vector These gene corstructs were inserted into an epresion vector nea Promoter Poly(A) consensus sequence A. tumefaciens Agrobactum umefaciens was transformed with the spresion vector Ti plasmid Bacterial chromosome 00 Recombination Infection Recombination trarsterned the gene constructs along with the promoter and polyAl sequences to the Ti plasmd Tobacco plents were inlected with the recombinant A tuberfaciens, and the Bt gere constructs were transfened to the plant crits
The at toin gere was souted from the bacterium Bacilus thuringiersis Chromosome Bacilius thuringiensis Bt toxin gene ard cloned vto E col. E coli plasmid The full-ength t gene and neo gene Bt gene ragments of the gene were gated to the ne gene -Ful-length Bt gene neo Cene Partial Bt genes constructs nee Promoter Restriction site PolyA) mee consensus sequence Expression vector These gene corstructs were inserted into an epresion vector nea Promoter Poly(A) consensus sequence A. tumefaciens Agrobactum umefaciens was transformed with the spresion vector Ti plasmid Bacterial chromosome 00 Recombination Infection Recombination trarsterned the gene constructs along with the promoter and polyAl sequences to the Ti plasmd Tobacco plents were inlected with the recombinant A tuberfaciens, and the Bt gere constructs were transfened to the plant crits
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
Question
. In Figure , what is the purpose of the neo+ gene that is attached to the Bt gene?
Transcribed Image Text:The at toin gere was
souted from the bacterium
Bacilus thuringiersis
Chromosome
Bacilius thuringiensis
Bt toxin gene
ard cloned vto E col.
E coli plasmid
The full-ength t gene and
neo gene Bt gene
ragments of the gene were
gated to the ne gene
-Ful-length Bt gene
neo
Cene
Partial Bt genes
constructs
nee
Promoter Restriction site
PolyA)
mee
consensus
sequence
Expression
vector
These gene
corstructs were
inserted into an
epresion vector
nea
Promoter
Poly(A) consensus
sequence
A. tumefaciens
Agrobactum
umefaciens was
transformed with the
spresion vector
Ti plasmid
Bacterial
chromosome
00
Recombination
Infection
Recombination trarsterned the gene constructs along
with the promoter and polyAl sequences to the
Ti plasmd
Tobacco plents were inlected with the
recombinant A tuberfaciens, and the Bt gere
constructs were transfened to the plant crits
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