The F integration site detemines the order of gene transfer in Hfrs thr thr thi thr thi pro pro pro gly lac lac his pur his pur his pur gal gal th gy his gal pur lac pro thr lac pur gal his gl tN thr pro pro lac pur gal his gly hi th H the thi pro thi pro lac lac his pur hi pur ga gai H Fartity tactor I Origin (first to enter) I Terminus (last to enter) gal his gy th thr pro lac pur gly his gal pur lac pro thr thi 312 FIGURE 5-14 The five E. col Hir strains shown each have different F plasmid insertion points and orientations. All strains have the same order of genes on the E col chromosome. The orientation of the F factor determines which gene enters the recipient cell frst. The gene closest to Two types of DNA transfer can take place the teminus enters last.

Biochemistry
6th Edition
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Reginald H. Garrett, Charles M. Grisham
Chapter29: Transcription And The Regulation Of Gene Expression
Section: Chapter Questions
Problem 2P: The Events in Transcription Initiation Describe the sequence of events involved in the initiation of...
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In Figure 5-14, which is the last gene to be transferred
into the F– from each of the five Hfr strains?

The F integration site detemines the order of gene transfer in Hfrs
thr
thr
thi
thr
thi
pro
pro
pro
gly
lac
lac
his
pur
his
pur
his
pur
gal
gal
th gy his gal pur lac pro thr
lac pur gal his gl tN thr pro
pro lac pur gal his gly hi th
H
the
thi
pro
thi
pro
lac
lac
his
pur
hi
pur
ga
gai
H Fartity tactor
I Origin (first to enter)
I Terminus (last to enter)
gal his gy th thr pro lac pur
gly his gal pur lac pro thr thi
312
FIGURE 5-14 The five E. col Hir strains
shown each have different F plasmid
insertion points and orientations. All strains
have the same order of genes on the
E col chromosome. The orientation of the
F factor determines which gene enters
the recipient cell frst. The gene closest to
Two types of DNA transfer can take place
the teminus enters last.
Transcribed Image Text:The F integration site detemines the order of gene transfer in Hfrs thr thr thi thr thi pro pro pro gly lac lac his pur his pur his pur gal gal th gy his gal pur lac pro thr lac pur gal his gl tN thr pro pro lac pur gal his gly hi th H the thi pro thi pro lac lac his pur hi pur ga gai H Fartity tactor I Origin (first to enter) I Terminus (last to enter) gal his gy th thr pro lac pur gly his gal pur lac pro thr thi 312 FIGURE 5-14 The five E. col Hir strains shown each have different F plasmid insertion points and orientations. All strains have the same order of genes on the E col chromosome. The orientation of the F factor determines which gene enters the recipient cell frst. The gene closest to Two types of DNA transfer can take place the teminus enters last.
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