The chart below represents the results (absorbance readings) of your Bradford Assay. Use the data and graph to determine the dilution of each well and concentration (in mg/ml) of the sample in each well of the microtiter plate.
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The chart below represents the results (absorbance readings) of your Bradford Assay. Use the data and graph to determine the dilution of each well and concentration (in mg/ml) of the sample in each well of the microtiter plate.
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- You obtained the following raw data when setting up a Bradford standard curve: BSA (mg/ml) Absorbancy 595nm 0 0.225 1 0.310 2 0.420 3 0.510 4 0.610 5 0.720 6 0.810 7 0.915 8 0.950 9 0.980 10 0.990 After blanking against a bradford-dH2O sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.570 was obtained. Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg / ml (up to 3 significant figures).You obtained the following raw data when setting up a Biuret standard curve: BSA (mg/ml) Absorbancy 540nm 0 0.158 1 0.210 2 0.260 3 0.305 4 0.360 5 0.410 6 0.455 7 0.510 8 0.530 9 0.550 10 0.554 After blanking against a biuret-dH2O sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.284 was obtained. Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg / ml (up to 3 significant figures).The following protein quantification utilizes Cu ions except fora. Lowryb. Biuretc.Bradford
- The following table provides data on three popular protein supplements. (Figures shown correspond to a single serving.) Protein (g) Carbohydrates (g) Sodium (mg) Cost ($) Designer Whey(Designer Protein) 20 6 90 0.80 Muscle Milk(Cytosport) 16 10 80 0.90 100%Whey Gold Standard(Optimum Nutrition) 24 3 210 1.00 You are thinking of combining Designer Whey and Muscle Milk to obtain a 3-day supply that provides exactly 200 grams of protein and 86 grams of carbohydrates. How many servings of each supplement should you combine in order to meet your requirements? Designer Whey _____servings Muscle Milk ______servings What will it cost? _______Given the following information, calculate the total activity in the undiluted protein sample. Activity of 1 ml of diluted sample = 0.5 Total volume of sample = 5 ml Dilution factor = 10 25 50.5 250 2.5Calculate protein concentration in unknown samples 1, 2, 3: Absorbance of Unknown 1 = 0.541 Absorbance of Unknown 2 = 0.85 Absorbance of Unknown 3 = 1.02 Standard Curve: Y = 0.0073x
- Using the data from the standard curve done previously, you record the absorbance of your extracted milk protein sample at 0.499 at 540nm. What is the mass of the protein in mg assuming you collected 10mL worth of the protein? Your standard curve should have a line of best fit with the equation y=0.0001x+0.0038.Using Bradford Assay, plot the standard curve and find the unknowns. Protein Concentration A595 of sample 0 0 3 0.220 6.5 0.383 12 0.667 18.8 0.930 24.2 1.090 Unknown 1 1.085 Unknown 2 0.708 Unknown 3 0.193If I had a standard with 2.0 mL of Bradford reagent then added 50μL of 250 μL/mL ; what would be the concentration of original protein?
- Given the Ramachandran Plot below, identify the protein components that could adopt the phi-psi angle combination indicated by the number 3.If a pediatric vitamin contains 1500 units of vitamin A per milliliter of solution, how many units of vitamin A would be administered to a child given 2 drops of a solution from a drpper calibrated to deliver 20 drops per milliar of solution?What is the approximate rate of change of A340 measured? i.e. ΔA340 / min = __________ What rate of change of A340 would you predict if 30 ml of the ADH solution was tested in the same way (i.e. half the amount of protein) ? i.e. ΔA340 / min = __________ What rate of change of A340 would you predict if 60 ml of a 0.5 μM ADH solution was tested in the same way? i.e. ΔA340 / min = __________ As well as writing your answers, explain your reasoning.