The consensus sequence of the protospacer adjacent motif (PAM) sequence in bacterial strain A is 5'-NGG-3, whereas in bacterial strain B the consensus PAM sequence is 5'-NGRRN-3. Calculate the frequency at which each of these PAM sequences will occur in DNA using N- any nucleotide and R purine base. Bacterial strain A: PAM sequence occurs once in every 16 nucleotides Bacterial strain B: PAM sequence occurs once in every 32 nucleotides Bacterial strain A: PAM sequence occurs once in every 32 nucleotides Bacterial strain B: PAM sequence occurs once in every 16 nucleotides Bacterial strain A: PAM sequence occurs once in every 32 nucleotides Bacterial strain B: PAM sequence occurs once in every 32 nucleotides Bacterial strain A: PAM sequence occurs once in every 16 nucleotides Bacterial strain B: PAM sequence occurs once in every 16 nucleotides

Biology: The Dynamic Science (MindTap Course List)
4th Edition
ISBN:9781305389892
Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Chapter19: Genomes And Proteomes
Section: Chapter Questions
Problem 1ITD: Below is a sequence of 540 bases from a genome. What information would you use to find the...
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The consensus sequence of the protospacer adjacent motif (PAM) sequence in
bacterial strain A is 5'-NGG-3, whereas in bacterial strain B the consensus PAM
sequence is 5'-NGRRN-3: Calculate the frequency at which each of these PAM
sequences will occur in DNA using N = any nucleotide and R = purine base.
Bacterial strain A: PAM sequence occurs once in every 16 nucleotides
Bacterial strain B: PAM sequence occurs once in every 32 nucleotides
Bacterial strain A: PAM sequence occurs once in every 32 nucleotides
Bacterial strain B: PAM sequence occurs once in every 16 nucleotides
Bacterial strain A: PAM sequence occurs once in every 32 nucleotides
Bacterial strain B: PAM sequence occurs once in every 32 nucleotides
Bacterial strain A: PAM sequence occurs once in every 16 nucleotides
Bacterial strain B: PAM sequence occurs once in every 16 nucleotides
Transcribed Image Text:The consensus sequence of the protospacer adjacent motif (PAM) sequence in bacterial strain A is 5'-NGG-3, whereas in bacterial strain B the consensus PAM sequence is 5'-NGRRN-3: Calculate the frequency at which each of these PAM sequences will occur in DNA using N = any nucleotide and R = purine base. Bacterial strain A: PAM sequence occurs once in every 16 nucleotides Bacterial strain B: PAM sequence occurs once in every 32 nucleotides Bacterial strain A: PAM sequence occurs once in every 32 nucleotides Bacterial strain B: PAM sequence occurs once in every 16 nucleotides Bacterial strain A: PAM sequence occurs once in every 32 nucleotides Bacterial strain B: PAM sequence occurs once in every 32 nucleotides Bacterial strain A: PAM sequence occurs once in every 16 nucleotides Bacterial strain B: PAM sequence occurs once in every 16 nucleotides
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