The flask where you grow the E. coli culture got exposed to UV rays, explain what kind of DNA damage can happen and how bacteria will repair the damage. Describe two DNA repair pathways.

Q: Transformation can be described as Polymerase chain reaction Sanger Sequencing Use of…

A: DNA is one of the genetic material present in the cell. The cell is modified by inserting the…

Q: The enzyme that proofreads the new strand of DNA, looking for and correcting mistakes as it…

A: DNA replication is the process in which the two identical replicas of DNA from one original DNA…

Q: The function of a restriction enzyme is to a. prevent the movement of DNA outside the nucleus b.…

A: Restriction enzymes:- these are the proteins and mostly isolated from prokaryotes. These enzymes…

Q: Which of the following mechanisms will remove uracil and incorporate the correct base?a) Direct…

A: The spontaneous changes in the DNA must be repaired to avoid mutations. The changes are repaired to…

Q: Which DNA polymerase in prokaryotes is involved in the replacement of primers with deoxynucleotides?…

A: DNA polymerase is the enzyme that catalyses the replication of DNA. DNA polymerase cannot synthesise…

Q: utline the four broad classes of chemical damage to DNA.

A: Epigenetic mechanisms are described in detail. Multiple biological processes that affect epigenetic…

Q: Describe and diagram the DNA damage done by UV radiation on a single strand of DNA. Describe in at…

A: The DNA molecules that encode its genome in human cells DNA damage is subdivided into two types:…

Q: Recombinant DNA construction involvesa) Cleaving DNA with restriction endonuclease and joining with…

A: Genetic engineering is the branch of biological science that can input alteration of DNA in an…

Q: Which of the following is true of DNA repair?

A: DNA repair is the process by which a cell identifies any damage in the DNA and rectifies it. It is…

Q: What will be the newly synthesized DNA from the template given? DNA Template 3 - CGGATGELLGTATAL -5…

A: DNA replication :-  It is the formation of a new DNA from a pre existing DNA.  The DNA so formed is…

Q: After you get your gene block, you do a restriction enzyme digest and ligation reaction with the…

A: Bacterial transformation is the transfer of free DNA/extrachromosomal DNA /plasmid released from a…

Q: The important excision mechanism that repair DNA damage induced by UV light are called Base excision…

A: The above given  statement is false . It is not known as base excision repair .  The statement given…

Q: When a geneticist or microbiologist uses ligase, what is the ligase being used for covalently…

A: DNA ligase is an enzyme that is used to join fragments of DNA by catalyzing the formation of…

Q: DNA polymerase replicates DNA producing a _______ strand.

A: Answer: DNA polymerase replicates DNA producing a new complementary strand. Explanation: Duplication…

Q: The following image shows how DNA can be damaged by UV light. In this reaction, UV light promotes…

A: Mutation: It is a heritable change in the genetic material of an organisms that give rise to…

Q: Recombinant DNA molecules are produced by incubating plasmids that have been broken open by a…

A: "Since you have asked multiple questions, we will solve the first question for you. if you want any…

Q: The enzyme ________ separates the TWO DNA strands of the double helix.     a. helicase   b. ligase…

A: Deoxyribonucleic acid (DNA) is the functional genetic information-carrying molecule present in cells…

Q: The action of ultraviolet radiation on DNA to induce mutation is the

A: A mutation is a change in a DNA sequence. It can result from DNA copying mistakes made during cell…

Q: The enzyme required to open up the double stranded DNA during replication and form the replication…

A: Replication Replication is a process by which the DNA synthesised exact copies of itself. This is…

Q: Which of the following is not depicted in the diagram attached? A. Okazaki fragment B. Replication…

A: Origin of replication is not depicted in the diagram. An origin of replication is a sequence of DNA…

Q: Below is a template strand (top) being replicated by DNA polymerase.   The black arrow is pointing…

A: DNA is the genetic material of almost all living organisms. It contains the information that is…

Q: Explain how X rays and UV light damage DNA.

A: Introduction  Mutation: any changes in the sequence in the genetic material which leads to disorders…

Q: . Which type of DNA repair is defective in severe combined immunodeficiency disease (SCID)?…

A: When there is defect in DNA damage repair , it leads to various human diseases .

Q: Which enzymes are involved in DNA replications? There are multiple answers. Helicase DNA polymerase…

A: DNA replication is the mechanism by which the eukaryotes and the prokaryotic DNA generates a copy of…

Q: After one round of PCR, one molecule of DNA consisting of two complimentary strands yields _____…

A: The polymerase chain reaction is a technique that helps to amplify the target DNA strand. The target…

Q: Which of the following would be a good application of plasmid transformation? 1) to make copies of…

A: The plasmid is a small, circular DNA molecule of two strands that differs from chromosomal cell DNA.…

Q: Cellular DNA replication uses the enzymes for the process while PCR uses thermal denaturation that…

A: DNA replication is the biological process of delivering two indistinguishable imitations of DNA from…

Q: Which enzyme was used to produce the molecule in the figure above? O ligase O DNA polymerase O RNA…

A: DNA cloning, is a process in which a piece of gene is inserted into the circular piece of DNA…

Q: Pyrimidine dimers in DNA may be repaired by in E. coli cells. (select all correct answers) Select…

A: The most well-known DNA lesion involving a single DNA strand is pyrimidine dimer. It is an…

Q: The type of DNA replication error illustrated in the diagram below is _______________________

A: Frameshift mutation .

Q: The process of transformation in bacteria would best be described as bacterial cells are infected by…

A: Answer. In 1928, griffiths experimented with virulence in Pneumococcus. During the 1920 Frederick…

Q: What is/are the attributes that make nucleotide excision repair (NER) and base excision repair (BER)…

A: Deoxyribonucleic acid (DNA) is a hereditary molecule that passes genetic information from one…

Q: Which of the following DNA repair systems may involve the removalof a segment of a DNA strand?a.…

A: Deoxyribonucleic acid (DNA) is a genetic material of most organisms. DNA contains the instructions…

Q: In DNA amplification, using the Taq polymerase, what is the maximum number of amplification cycles?…

A: Introduction In the field of molecular biology, however, cloning is viewed at a genetic molecular…

Q: What enzyme replaces the RNA primers on the Okazaki fragments on the lagging strand? DNA Pol II

A: DNA replication is the process by which DNA duplicates itself. One of the most important feature of…

Q: AKS 5b: Which model accurately represents the semi-conservative nature of DNA replication? * AA AA…

A: DNA( deoxyribonucleic acid) is the double-stranded molecule that is the genetic material in most…

Q: Give the name of the enzyme that catalyzes each of the following reactions:(a) Makes a DNA strand…

A: Transcription is the first of several steps of DNA based gene expression in which a particular…

Q: The DNA polymerase involved in base excision repair isa) DNA polymerase αb) DNA polymerase βc) DNA…

A: DNA polymerase is an enzyme that synthesizes DNA molecules from deoxyribonucleotides, the building…

Q: Which of the following enzymes has the highest functional error?  a) DNA Polymerase I b) DNA…

A: The proofreading activity of enzymes helps in reducing the number of errors. Proofreading activity…

Q: What type of spontaneous change to DNA is caused by UV light? deamination

A: Deoxyribonucleic acid (DNA) is a polymer made up of two polynucleotide chains that coil around each…

Q: This diagram shows the functions of different enzymes during DNA replication. Which label would…

A: Cells are the basic unit of life and life originated from nucleic acid according to several theories…

Q: Guanine was oxidized into 8-oxoguanine, which process below will ensure the integrity of the DNA?…

A: DNA repair system specifies a collection of processes, through which a cell identifies and corrects…

Q: ____ cut(s) DNA molecules at specific sites.   a. DNA probes   b. Restriction enzymes   c. DNA…

A: The DNA molecule is made up of two strands that coil around each other to form a double helix…

Q: Tell Me Why - Why don't scientists who work with recombinant DNA know all the long-term effects of…

A: Recombinant DNA technology is a significant scientific advancement that has made human life…

Q: DNA polymerase l moves toward the direction of replication fork creating Okazaki Fragments. * True…

A: Most living organisms that are well defined in terms of that they have DNA as their genetic…

Q: In the figure, which ketter represents DNA polymerase 1 D AR AA BB C.C DD EE

A: Polymerase A kind of enzyme that make DNA or RNA from pre-existing DNA or RNA.

Q: DNA polymerase III requires a(n)__________ for synthesis of DNA to occur  A- DNA template  B-RNA…

A: DNA polymerase III binds with the primers on the leading and lagging strand and synthesize new DNA…

Q: Prokaryotes use methylation as a major mechanism of DNA protection because (A) methyl groups include…

A: Prokaryotic cells do not have a distinct nucleus but are situated within a cell, called the…

Q: What kind of DNA damage is depicted in this picture: H2D но. H.N Bar strand breakage O base deletion…

A: DNA damage is defined as the deterioration of the bases or the chemical addition that modifies the…

Tissue regeneration & repair

Tissue regeneration involves the restoration of tissue components by regrowth of damaged tissues. In contrast, tissue repair involves patching of injured tissue rather than restoring it. It is the proliferative capacity of the cells that decides the amount of regeneration against the repair that would occur in the tissue. Both regeneration and repair depending on the duration of the injury and response to the inflammation.