The Lineweaver-Buck plot is said to give undue weight to measurements made at low substrate concentrations, when the results are likely to be more accurate?

Q: Briefly explain the principle of Bradford Method of Total Protein Estimation

A: Asked : Principle of Bradford Method : Total Protein Estimation.

Q: Calculate the percentage efficiency of extraction of phycocyanin in sample 3, if the amount of…

A: Given Values: Amount of phycocyanin in sample = 119.2 mg Amount of phycocyanin extracted from sample…

Q: In the work-up, the reaction mixture is neutralized with 3M sulfuric acid to a final pH of 1-2. Why…

A: PH of a substance or reaction is defined as the negative logarithm of the hydrogen ion…

Q: When eluting your analyte of interest using HPLC, gradient elution can be used. How does Gradient…

A: HPLC is expanded as High Performance Liquid Chromatography. It helps in excluding out the analyte in…

Q: measured in the experiment is different from the Tm predicted from Marmur and Doty equation. please…

A: In 1962 Marmur and Doty discovered a linear relation between the base composition of DNA expressed…

Q: DISCUSS THE TWO GENERAL METHODS EMPLOYED IN OBTAINING FATS AND OILS FROM SOME OF THE USUAL SOURCES…

A: Introduction Fats and oils are the basic macromolecules present in many usual or daily sources…

Q: the cell potential table found in the Reference Material,  why do you get holes in your aluminum…

A: The natural process that results in the conversion of a reactive metal into a stable metal by…

Q: Friedewald equation is an indirect method, used to estimate LDL from the measurement of total…

A: Low-density lipoprotein cholesterol (LDL-C) remains a major contributor to plaque formation and…

Q: aelis-Menten constant an be determined using the Lineweaver-Burk plot and is equal to twice the…

A: Mechaelis-Menten Constant: - Km is used in the enzyme kinetics, it plays very important role in the…

Q: Please discuss the value of international normalized ratio (INR) as a test. Why do you think this is…

A: PT test is the normal blood test conducted to record the time taken for blood to clot.

Q: How to graph a scatter plot /X-Y plot of ph and absórbance. pH is the independent variable (x-axis)…

A: pH of tube A400 after 5 minutes           3   0.008     5   0.371     7   0.546     9…

Q: In both experiments, affinity purification and ion exchange (IEX) columns, we have loading, washing,…

A: Chromatography is a scientific technique for separating a mixture into its constituent parts. It has…

Q: In glucose estimation using Benedict's Reagent, what is/are the chemical reaction/s that resulted in…

A: Benedict reagent contains potassium thiocyanate, copper sulphate and sodium carbonate.  This reagent…

Q: Determine the value of K, for an competitive whose data results in the linear equation

A: The inhibitor constant, Ki, is an indication of how potent an inhibitor is; it is the concentration…

Q: Explain the purpose of the two control solutions

A: Ans: Control solution: The solution which mimics the blood and is used for testing the accuracy of a…

Q: calculate the rate of weight change over this 30-minute period Write your answer in standard…

A: Introduction: Carbohydrates are produced by the green plants by the process of photosynthesis.They…

Q: Cupations Car i'ons. Itiyou have been providea with a new enyme e xplain related plot by which a…

A: Enzymes consist entirely of amino acids which are linked with each other by a peptide bond.…

Q: Below is a simple set of weights obtained while immersing potato slices in various sugar solutions…

A: Note: As per Bartleby Guidelines For Remaining Answers Please Repost The Question. Introduction:…

Q: Discuss the limitations of the lineweaver-burk plot and suggest alternative secondary plots and…

A: An enzyme is a biocatalyst that increases the rate of chemical reaction without itself being changed…

Q: Why is a Lineweaver–Burk plot useful in analyzing kinetic data from enzymatic reactions?

A: Gerenally, The Lineweaver–Burk plot was wide accustomed to confirm vital terms in protein kinetics,…

Q: What are the possible errors that could occur during the titration experiment to determine acetic…

A: Titration is the analytical  method which  determine the unknown concentration of the chemical…

Q: ich of these statements best describes the results shown in the graph?

A: This experiment in the form of graphs  shows the relationship between air temperature and the…

Q: You obtained the following raw data when setting up a Bradford standard curve: BSA (mg/ml)…

A: The Bradford protein assay is a colorimetric assay using the Bradford reagent (acidified Coomassie…

Q: The Bradford reagent gives a linear response only from 0.1 mg/mL to 1.4 mg/mL of protein…

A: There are several biochemical tests that are used to determine the concentration of protein in a…

Q: or each solution, calculate the rate of weight change over this 30-minute period.  *****Please Show…

A: Given the weight of potatato slices immersed in 0M, 0.4M 0.6M, 0.8M and 1 M sucrose solution over 30…

Q: How to prepare a 12 mL solution of 1 mM NaCl in deionized water if your precision balance has a…

A: Molality or molar concentration is the amount if substrate dissolved in a certain mass of solvent.…

Q: Activity results of this experiment to show the optimum pH for this reaction USAUG COU

A: The absorbance depicts the activity of an enzyme. The ph is defined as the negative logarithm of…

Q: In the Isoelectric Precipitation of Proteins: Casein from Milk -  What would happen to the…

A: Casein is a protein, which is present in milk. These are present in the milk in the form of calcium…

Q: Explain the observed results of unboiled starch –saliva mixture with iodine solution and fehling's…

A: Starch or amylum is a polymeric sugar comprising of various glucose units joined by glycosidic…

Q: Discuss the advantages and disadvantages of Protein efficiency ratio(PER)

A: Protein efficiency ratio (PER) is the weight gained by a test subject divided by its intake of a…

Q: Why are buffers important in biochemical experiments?  Please explainw ell

A: Buffer : solution which resists any change in pH via the addition of any acidic/ the basic…

Q: You want to prepare 500 mL of the macronutrient and micronutrient stock solutions which have 100X…

A: MS medium is a culture medium for plant cells. Murashige and Skoog (MS) medium was formulated by two…

Q: Differentiate Wintrobe from Westergreen regarding the accuracy of the test in a tabulated order.

A: As per our policy, We are answering only question 1. For the rest of the questions please repost.…

Q: You put 50 uL of 0.1 mg of protein into a 1 cm pathlength cuvette containing 3 mL of LDH reaction…

A: Absorbance studies are used to calculate the concentration of a sample. It is based upon the Beer…

Q: For which purpose the method of measuring biochemical demand is used?

A: Most natural waters contain small quantities of organic compounds. Aquatic microorganisms have…

Q: 1. Acids and Bases a. How reliable are the indicators (red cabbage and phenolphthalein) in the…

A: Hi! Thank you for the questions. As you have posted multiple questions, I will be answering the…

Q: what are the complications or the low-reliability in determining the value of KM using graphical…

A: Michalis-Menten equation was discovered in 1913 by Michalis and Menten. According to Michalis-Menten…

Q: Show calculations for making 10 mls each of the following dilutions of sucrose from the 0.30 M stock…

A: Dilution is defined as a term that refers to the solution that is made by adding solvent to a…

Q: List the 6 assumptions by Henri and Michaelis-Menten in describing the relationship between initial…

A: Victor Henri was the first one who tried to relate the velocity or rate of an enzyme catalyzed…

Q: Calculate the percentage efficiency of extraction of phycocyanin in sample y, if the amount of…

A: Percentage efficiency of extraction = OutputInput×100

Q: Compute for the CFU/ml of drinking water. Is this Passed or Failed based on the Philippine National…

A: Drinking water is the water that is used in drink or food preparation. The potable water is that…

Q: The absorbance values for different BSA standards.  How can the protein concentration be calculated?…

A: The Bradford protein assay is used to measure the total protein in a given solution. It is a…

Q: acetate buffer works on fumarase activity but tris-maleate buffer does not

A: Fumarase is an enzyme that catalyzes the reversible conversion of fumarate to malate in the TCA…

Q: If the enzyme lactase has a Vo of 0.40 mM per minute when [S] = 1.25 mM, and a Vo of 1.0 mM per…

A: The Lineweaver-Burk equation is obtained by taking the reciprocal of the Michaelis-Menten equation.…

Q: You put 50 uL of 0.1 mg of protein into a 1 cm pathlength cuvette containing 3 mL of LDH reaction…

A: Enzymes are the biocatalysts that decrease the activation energy in turn increasing the rate of…

Q: Below is a simple set of weights obtained while immersing potato slices in various sugar solutions…

A:

Q: Discuss the advantage and disadvantage of Wijs method of iodine value determination

A: Iodine value: The iodine value of an oil/fat is the number of grams of iodine absorbed by 100g of…

Q: Make a schematic diagram of the procedure of Barfoed's Test which is also a test for reducing

A: Carbohydrates are polyhydroxy aldehydes or ketones which are associated with reducing properties.…

Metabolic pathways

Metabolism is defined as the group of life-supporting, enzyme-mediated chemical reactions in organisms. A metabolic pathway is a set of these chemical reactions that involve anabolism (synthesis of complex biomolecules) and catabolism (breakdown of biomolecules into small molecules) that occur inside a cell. Metabolites are the reacting substances, products, and intermediate substances of an enzymatic process that are altered by a series of chemical events catalyzed by enzymes.