The sequence below (A) was read from the autoradiogram (B).(A)5' TGTACAACTTTTACTTAGGGCCGTGACACCTAAAG. 3'(B)Negative endACGTPositive endC.Compare the sequence to the autoradiogram. How is the sequence read? Explain your ansd.Suppose that you want to express the correct protein product of an eukaryotic gene in a bacterial cell using a plasmidvector. What single sequence related factor must your consider in the cloning experiment?

Name: The sequence below (A) was read from the autoradiogram (B).(A)5' TGTA
Uploaded: 2024-03-20T06:42:41.000Z
Channel: Bartleby
Description: The sequence below (A) was read from the autoradiogram (B).(A)5' TGTACAACTTTTACTTAGGGCCGTGACACCTAAAG. 3'(B)Negative endACGTPositive endC.Compare the sequence to the autoradiogram. How is the sequence read? Explain your ansd.Suppose that you want to

Note: since you have asked multiple unrelated questions, as per the honor code we are answering the…

Science

Biology

The sequence below (A) was read from the autoradiogram (B). (A) 5' TGTACAACTTTTACTTAGGGCCGTGACACCTAAAG. 3' (B) Negative end ACGT Positive end Compare the sequence to the autoradiogram. How is the sequence read? Explain your answer.

The sequence below (A) was read from the autoradiogram (B). (A) 5' TGTACAACTTTTACTTAGGGCCGTGACACCTAAAG. 3' (B) Negative end ACGT Positive end Compare the sequence to the autoradiogram. How is the sequence read? Explain your answer.

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter19: Genomes And Proteomes

Section: Chapter Questions

Problem 1ITD: Below is a sequence of 540 bases from a genome. What information would you use to find the...

See similar textbooks

Similar questions

Refer to the DNA sequence provided: 3’ -TACTGAAGCGGCAGCCCCGCATGAGTAGACCTTACT-5’ a. What is the mRNA transcript of the anticoding strand of the DNA model? b. What is the amino acid sequence of the polypeptide chain that will be translated from the mRNA in (a)?
The protein encoded by the cystic fibrosis gene is 1480amino acids long, yet the gene spans 250 kb. How is thisdifference possible?
For the following sequence, what is the Tm? 5'-AGCTACGATCAGGTCA-3'
Given the following DNA sequence: 5’-ATGCGGCCAAGGTCAGAGTGACA-3’ a) If this DNA strand represents the “Sense Strand” of DNA, what would be the RNA sequence? b) If this DNA strand represents the “Antisense Strand” of DNA, what would be the RNA Sequence? c) What would be the other strand of DNA?
Based on the alignment of these protein sequences, which [pairs] of the genes appear to be most similar to each other? And give reasons why?
Below is a sequence of 540 bases from a genome. What information would you use to find the beginnings and ends of open reading frames? How many open reading frames can you find in this sequence? Which open reading frame is likely to represent a protein- coding sequence, and why? Which are probably not functioning protein-coding sequences, and why? Note: for simplicitys sake, analyze only this one strand of the DNA double helix, reading from left to right, so you will only be analyzing three of the six reading frames shown in Figure 19.4.
If an extra nucleotide is inserted in the first exon of the beta globin gene, what effect will it have on the amino acid sequence of the globin polypeptides? Will the globin most likely be fully functional, partly functional, or nonfunctional? Why?
The DNA-binding domain of each CREB protein subunit recognizes the sequence 5′–TGACGTCA–3′. Due to random chance, how often would you expect this sequence to occur in the human genome, which contains approximately 3 billion base pairs? Actually, only a few doze genes are activated by the CREB protein. Does the value of a few dozen agree with the number of random occurrences expected in the human genome? If the number of random occurrences of the sequence in the human genome is much higher than a few dozen, provide at least one explanation why the CREB protein is not activating more than a few dozen gene Actually, only a few doze genes are activated by the CREB protein. Does the value of a few dozen agree with the number of random occurrences expected in the human genome? If the number of random occurrences of the sequence in the human genome is much higher than a few dozen, provide at least one explanation why the CREB protein is not activating more than a few dozen gene
in the human gene for the beta chain of hemoglobin, the first 30 nucleotides in the amino acid coding region is represented by the sequence 3'TACCACGTGGACTGAGGACTCCTCTTCAGA-5'. What is the sequence of the partner strand? If the DNA duplex for the beta chain of hemoglobin above were transcribed from left to right, deduce the base sequence of the RNA in this coding region.
Below is a sequence of DNA. 5'-ttaccgataattctctctcccctcttccatgattctgattaaagaaggcgagaacgaaactatttgttaatacc-3' Using the one letter code for Amino Acids, what is the predicted AA sequence of the shortest ORF (from N to C-terminal end)? Using the one letter code for Amino Acids, what is the predicted AA sequence of the longest ORF (from N to C-terminal end)?
In the procedure shown, why was it necessary to link thecoding sequence for the A or B chains to the sequence forβ-galactosidase? How were the A or B chains separated fromβ-galactosidase after the fusion protein was synthesized in E. coli?
(a) How many activation cycles are needed for a protein with 150 amino acids? (b) How many initiation cycles are needed for a protein with 150 amino acids? (c) How many elongation cycles are needed for a protein with 150 amino acids? (d) How many termination cycles are needed for a protein with 150 amino acids?