The simple form of |Hoff equation is: Il = [B]RT In this equation the [B] is the molar concentration of solute. So: m [B] M,V Where c, the mass concentration of the solute is in the total volume of solution and Ma is the molar mass of the solute. This equation can be replaced in the previous one to get: RT II =; In this equation molar mass of given solute can be detemined from the slope of the II vs cg plot. This equation applies only to solutions that are sufficiently dilute to behave as ideal-dilute solutions. In the case of non-ideal solutions, however, the extended formula is: Il = [B]RT{1+ k. [B] + n. [B]² + .-.} Biological macromolecules dissolve to produce solutions that are far from ideal, but we can still calculate the osmotic pressure by assuming that the van't Hoff equation is only the first term of a lengthier expression: II [B]RT(1+b. [B])

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The simple form of |Hoff equation is:
II = [B]RT
In this equation the [B] is the molar concentration of solute. So:
n
m
[B] =
v MV
= cg /MA
Where c, the mass concentration of the solute is in the total volume of solution and M, is the molar mass of the solute. This equation can be
replaced in the previous one to get:
RT
II =
MA
In this equation molar mass of given solute can be detemined from the slope of the II vs Cz plot. This equation applies only to solutions that are
sufficiently dilute to behave as ideal-dilute solutions. In the case of non-ideal solutions, however, the extended formula is:
II = [B]RT{1+ k. [B] + n. [B]² + ...}
Biological macromolecules dissolve to produce solutions that are far from ideal, but we can still calculate the osmotic pressure by assuming that
the van't Hoff equation is only the first term of a lengthier expression:
II [B]RT(1+ b. [B])
II
= RT + bRT. [B]
[B]
II
= RT + bRT./M.
*/Ma
п
RT ÞRT
Ca
MA
MA
In this equation molar mass of given biomolecule can be detemined from the intercept of
vs. Cs plot.
The osmotic pressures of solutions of a protein at 25°C were as follows:
C5 (g/L)
0.5
1.0
1.5
2.0
2.5
П (Ра)
40.0
110
200
330
490
What is the molar mass of the protein?
Transcribed Image Text:The simple form of |Hoff equation is: II = [B]RT In this equation the [B] is the molar concentration of solute. So: n m [B] = v MV = cg /MA Where c, the mass concentration of the solute is in the total volume of solution and M, is the molar mass of the solute. This equation can be replaced in the previous one to get: RT II = MA In this equation molar mass of given solute can be detemined from the slope of the II vs Cz plot. This equation applies only to solutions that are sufficiently dilute to behave as ideal-dilute solutions. In the case of non-ideal solutions, however, the extended formula is: II = [B]RT{1+ k. [B] + n. [B]² + ...} Biological macromolecules dissolve to produce solutions that are far from ideal, but we can still calculate the osmotic pressure by assuming that the van't Hoff equation is only the first term of a lengthier expression: II [B]RT(1+ b. [B]) II = RT + bRT. [B] [B] II = RT + bRT./M. */Ma п RT ÞRT Ca MA MA In this equation molar mass of given biomolecule can be detemined from the intercept of vs. Cs plot. The osmotic pressures of solutions of a protein at 25°C were as follows: C5 (g/L) 0.5 1.0 1.5 2.0 2.5 П (Ра) 40.0 110 200 330 490 What is the molar mass of the protein?
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