Data from enzyme inhibition are used to determine a Kmapp and Vmax PP. Comparison of thesevalues with assays run without inhibitor are used to understand how the inhibition is occurring.This is useful for better understanding the active site as well as the practical aspect ofpharmaceutical drugs. Below are idealized Line-Weaver Burke plots of different types ofinhibitors.ComnetitiveUncomnetitiveMixed+Inh+Inh4InhAnhInhAnh[S][S][S]a. How does the value of Vmax for the enzyme compare to the Vmax PP of the inhibited enzymefor:i. Competitiveii. Uncompetitiveiii. Mixedb. How does the value of Km for the enzyme compare to the Km PP of the inhibited enzyme for:i. Competitiveii. Uncompetitiveiii. Mixedc. For each situation in Model 1, consider an inhibitor that is better than the one shown onthe graph. Answer the following questions for each type of inhibition:i. How would the KmPP change?ii. How would the Vmax PP change?

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Answered: Uncompetitive Mixed +Inh +Inh AInh Tnh…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Uncompetitive Mixed +Inh +Inh AInh Tnh Anh Anh [S] [S] a. How does the value of Vmax for the enzyme compare to the Vmax "PP of the inhibited enzyme -15