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- What is gradient elution? a. Variation of the column temperature during elution b. Variation of the stationary phase composition during elution c. Variation of the mobile phase composition during elution d. Variation of the mobile phase flow rate during elutionWhen the distribution coefficient increases, the extraction efficiency increases or decreasesFind the weighted adjacency matrix of the following direted graph and represent that as a CSR-marix.
- I have a question with the answer but I don't really understand it. Can you please explain the logic in this question & answer? For example, the hormone estradiolhas a partition coefficient of 3.2 between 1-octanol and water. How many extractions would need to be completed to recover > 99% from 250 mL of saturated solution using 100 mL of 1-octanol per extraction? The variables given are listed below along with the solution. In this scenario, it would require 5 extractions to recover > 99% of estradiol! Typically extractions are carried out only 2 or 3 times because the partition coefficient is usually larger than 3.2 and there are equal amounts of organic solvent and the aqueous layer.How long is a macrocentury, if a lecture period (50.0 min) is close to 1.00 microcentury?Why does the efficiency (decreased plate height) of liquid chromatography increase as the stationary phase particle size is reduced?
- What effect will the following have on plate height (H). Make sure to explain your full and complete reasoning. Increasing stationary phase thickness. Reducing the rate of sample injection. Increasing mobile phase flow rate. Decreasing temperature. Reducing stationary phase particle size.Is standardization against primary standard required for controlled potential coulometry ?a. Briefly explain why internal standardization method is useful inanalytical chemistry?b. Why does a response factor of an instrument’s detector need to becalculated?
