What major impact does reducing film thickness of stationary phase in a wall-coated capillary column have on the separation efficiency (i.e., band broadening) in GC: Shorter tr (retention time) O Lower C-term Lower resolution O Higher A-term Higher B-term

Q: How can l exploit the mobile phase composition to optimize my HPLC separation?

A: The technique which is commonly used in laboratory for the separation of components of mixture is…

Q: List the variables that lead to band broadening in chromatography?

A: Band broadening in chromatography is the broadening or dispersion of a chromatographic peak during…

Q: Focusing Lenses Radiation Source Wavelength Selector Detector Atomizer Signal Processor Amplifier…

A: 5) The capillary column are more efficient than packed columns in GC.

Q: Suppose that a solution has an absorbance of 0.226 at a wavelength of 450 nm.  If the concentration…

A: Given the absorbance of the solution, A = 0.226 Concentration of the solution, C = 24 μM  path…

Q: 9. (a) Derive an expression for Langmuir adsorption isotherm for surface reactions with and without…

A: The answers are given below:

Q: What are the advantages of a dropping-mercury electrode in polarography? Why is polarography used…

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Q: Explain why the retention times for both carvone isomers are the same.

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Q: How can band broadening be reduced in HPLC Eddy Diffusion? A. use smaller particles for stationary…

A: HPLC is based on the principle where the sample (analyte) is distributed between a mobile phase…

Q: What adsorbents are typically used in TLC? On a TLC, a compound traveled 3.5 cm when the solvent…

A: Thin layer chromatography is the technique that is used to separate or purify the compound from a…

Q: Explain how XPS can be used to identify the compositions of the material in the layers below the…

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Q: The image below shows van Deemter curves for five different HPLC columns used under the same…

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Q: +3 The absorbance of a 1.05x10° M Fe" solution was determined at 580 nm using a 2.50-cm cell. The…

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Q: Estimation of Rhodamine B in Wastewater-UV-VIS Spectrophotometry

A: Interpretation - Explain the estimation of Rhodamine -B in wastewater -UV-vis spectrophotometer -…

Q: b) Helen performed an experiment with Fe(SCN)²+ complex at 580 nm. Calculate the percent…

A: Given: concentration of Fe(SCN)2+, c = 4.47 x 10-5 M length of cuvette = 1 cm  molar absorptivity, ε…

Q: Is the retention of particles related to the RSS or the relative supersaturation of the solution?…

A: Given: Retention of particles is related to  relative supersaturation of solution. To find: whether…

Q: Magnetic resonance contrast agent relaxivity is defined as the proportionality constant relating the…

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Q: Which of the following statements DOES NOT describe the real limitation of Beer's Law? The…

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Q: What are the different tetrabasic forms of EDTA? Describe their individual reactivities.

A: The structure of EDTA: EDTA (Ethylenediaminetetraacetic acid) is an amino polycarboxylic acid. The…

Q: Choose the correct option. Which one of following techniques is used as fractionation as well as…

A: Molecular weight is the mass of one mole of substance. Units of molecular weight is grams per mole.…

Q: Cite problems why t

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Q: ___________ is a method which can be used to separate enantiomers. Column chromatography Proton…

A: Given: Enantiomers To find: Method use to separate enantiomers Solution: Enantiomers are those…

Q: II B C D What is the design of figure Il? double beam spectrophotometer diode array…

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Q: Mass spectrometry and UV/Vis absorbance spectroscopy can both be used as letectors for liquid…

A: Note: Since you have posted multiple independent questions in the same request, we will solve the…

Q: 3) Figure below shows an x-ray diffraction pattem for single crystal scandium nitride (ScN), having…

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Q: (A) Draw a sketch of the expected chromatrogram (peaks, broadening, relative elution times, etc.)

A: Given: A) To sketch the expected chromatogram.

Q: Describe two applications of Potentiometric analysis

A: Potentiometric analysis is a process in which the potential of a substance is measured with respect…

Q: What are the disadvantages of uses the Thick Layer stationary phase in Thin Layer Chromatography…

A: What are the disadvantages of uses the Thick Layer stationary phase in Thin Layer Chromatography…

Q: Which of the following statement is incorrect? The quality of grating of the monochromator affects…

A: In the Atomic Absorption Spectroscometer, the following instrumentation is used and their uses are…

Q: The absorbance values ​​observed during the determination of the complex stoichiometry with the Job…

A: A compound is formed when electrons are lost (or) electrons are gained. The number of electrons that…

Q: 1. 2. Silica 3. the column frits. 4. Surface metal ions act as multiple polar groups. is less stable…

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Q: 11. (8pts) how can silica gel Fsa TLC plates can be visualized ( give two methods? :

A: In the given question we have to visualise the F254 TLC plate, there are two method by which we can…

Q: Inorg. 2.4: The Microscale Separation of Divalent Metal Ions (Ni²+ and Co²+) by Ion-Exchange…

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Q: TGA analysis

A: Ans. Thermogravimetric analysis or TGA techniques helps in determination of thermal stability of…

Q: Measurement of adsorption of 40 mL of solution containing Hg by atomic absorption spectrometer…

A: Measurement of adsorption of 40 mL of solution containing Hg by atomic absorption Spectrometer…

Q: What is the benefit of cooling down the separation capillary in a CE system to low temperature?

A: Answer - Capillary electrophoresis - Capillary electrophoresis is a family of electrokinetic…

Q: Band broadening in chromatography is .a O inversely proportional to the column efficiency .b O…

A: Since you have asked multiple questions, we will solve the first question for you. If you want any…

Q: Peak or band broadening is more likely to occur in: gradient elution or isocratic elution?

A: Gradient elution and iscratic elution are two different types of liquid chromatography. Both…

Q: The accuracy of a spectrophotometer can be evaluated by preparing a solution of 60.06-ppm K2Cr2O7 in…

A: Molar absorptivity A= ɛ ×C×l ɛ=A/CL A=absorption C=concentration l=length

Q: Three isotropic elements are placed along the y-axis at y = -d, y = 0, and y = d. Their excitations…

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Q: Briefly explain the principle behind the following techniques: (a) ion-exchange chromatography (b)…

A: Question is based on chromatography techniques:

Q: In general, we want to have absorptions less than or equal to 1. Given that an ethanol solution of…

A: • According to Beer-Lambert law when a monochromatic light having intensity I is passed through the…

Q: 12 The following results were obtained in an experiment to determine spermine by high performance…

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Q: What is the molar absorptivity of a 2.50 x 10-4 M solution if its absorbance at 550 nm is 0.500? The…

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Q: 8. Why are higher resolution monochromators found in ICP-OES spectrometers than in flame AAS…

A: Spectrometers are the instruments helpful in observing and analyzing the substance in the given…

Q: . Reversed phase HPLC refers to the condition of stationary phase and a mobile phase of separation.…

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Q: 2. Account for the versatility of thin-layer liquid chromatography.

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Q: Compound Unretained solutes Tr (min) W (min) 1.19 8.04 0.15 B. 8.26 0.15 8.43 0.16

A: Chromatography resolution implies how well two peaks gets separated. It depends on coloumn length.

Q: Match the following chromatographic techniques with these corresponding principles of separation…

A: ✓chromatography, technique for separating the components, or solutes, of a mixture on the basis of…

Spectroanalytical Methods

Spectroanalytical methods measure the wavelength and intensity of radiations that are either emitted or absorbed by a molecular or atomic species. These methods are useful for evaluating the electronic or atomic arrangement of the molecules of a given analyte.