What summary can be drawn from the stab slant and surface inoculation.

Q: How might storage of the coconut water samples at room temperature after collection and before…

A: Under the regulated conditions of the laboratory when microbial cells are allowed to grow and…

Q: What is the purpose of flame-sterilizing the mouth of the test tube and the side of the Petri dish…

A: Flame is an excellent way of cleaning a surface from contaminating organisms. Instant high heat…

Q: What is the purpose of flame sterilizing the inoculating loop or needle before and after using it?…

A: These questions are related to the microbiology field. Microbiology is a branch of science that…

Q: methods of sterilization

A: Sterilizationis  the statistically complete destruction of all microorganisms including the most…

Q: What are the physical methods of microbial control

A: Control of microorganisms is fundamental to forestall the transmission of illnesses and disease,…

Q: Describe the steps you would take to isolate, cultivate, and identify amicrobial pathogen from a…

A: The techniques used in the identification of microbes can be termed as the six "I's": inoculation,…

Q: What is the volume of broth that can be picked up by loopful of sterile inoculating loop?

A: INOCULATING LOOP It is a simple tool mainly used  to pick up and transfer a small sample from a…

Q: Serial dilution is recommended prior to pour and spread plate. Why is this so?

A: Pour Plate: it is a technique in which A plate made by combining the inoculum which is cooled but…

Q: What will pose an ethical issue in these procedures are performed - sterilization?

A: Sterilization is a permanent method of family planning. It is one of the most common method of…

Q: Does a positive presumptive test mean that the water is absolutely unsafe to drink?

A: In microbiology, the presumptive test is used to find out whether gas-forming lactose fermenters…

Q: Why is the membrane filter method preferred to the most probable numbermethod (MPN) for the…

A: Most probable number (MPN) is a method used to estimate the numbers of bacterial cells in a water…

Q: Discuss two mechanized /automated process that are used in modern broiler production?

A: Poultry farming is defined as a term for rearing and keeping of birds such as fowl, duck and hen for…

Q: Is the Triple-Sugar Iron Agar (TSIA) a complex or defined medium? Explain based on its composition.…

A: A growth medium is a also known as a culture medium which can be solid, liquid, and even…

Q: How do we prepare smear

A: The preparation of a smear is required for many laboratory procedures, including the Gram-stain, for…

Q: Is the Peptone Iron Agar (PIA) a complex or defined medium? Explain based on its composition. Is the…

A: Depending on their composition, media can be classified as defined and undefined media. Defined…

Q: Explain why the following step are necessary during subculturing:  Flaming the neck of…

A: Aseptic technique-Aseptic technique should be observed whenever microorganisms are transferred from…

Q: Briefly explain the shake-tube inoculation

A: Inoculation is the key process in the microbiology in which inoculum is extracted.

Q: What is an E-test strip, and what is it used for in the microbiology laboratory? Draw a diagram of…

A: Microbiology is a branch of science which deals with the study of microscopic organisms such as…

Q: In what ways is the membrane filter method for coliform detection superior to the most probable…

A: Coloriform are group of bacteria present in the feces of several warm-blooded animals and human.…

Q: Does a positive presumptive test mean that the water is absolutely unsafe to drink? Explain

A: Water is tasteless, transparent, inorganic. Colorless, and odorless chemical substance. Its chemical…

Q: What is the principle behind sterilizing media before use and before disposal

A: Microorganisms may be beneficial or harmful. The harmful microorganism causes many infections which…

Q: Describe situations that require high-level disinfection and thosefor which medium- or low-level…

A: Step 1 Disinfection involves the use of a chemical sterilant or agent to eliminate virtually all…

Q: Explain why it is important to use only a small amount of bacteria when preparing a smear

A:

Q: How is air contamination prevented when an inoculating loop is used to introduce or take a bacterial…

A: Biotechnology is a branch of biology, including the use of living organisms to produce products.…

Q: How does sterilizer spray work from drones?

A: Sterilization is a process by which a material is completely devoid of microorganisms like bacteria,…

Q: Which test require a straight line inoculation? Choose all that apply

A: Answer: STRAIGHT LINE TECHNIQUE : It is the streaking technique of bacterial colony over the agar…

Q: Loop with bacterial inoculum was subjected to flame sterilization prior to streaking on agar plate.…

A: Bacterial colonies are cultured in laboratories for various purposes. During bacterial culture, it…

Q: Using aseptic techniques, list the general procedure of inoculating a broth from a plate culture.

A: Aspetic techniques that you should follow during inoculating a broth from a plate culture are:…

Q: Which of the following tests can be used to distinguish between Enterobacter and Pseudomonas…

A: Introduction  Small, single-celled organisms are known as bacteria. Nearly every place on Earth…

Q: How does Pseudomonas aerogenosa contribute to the virulance of the pathogen?

A: Pseudomonas aeruginosa is a bacterium which is liable for serious infectious diseases, chronic…

Q: Before using the API® Staph System, what tests should be performed on bacteria to confirm that they…

A: API Staph (analytical profile index) is a systematized procedure used for the identification of the…

Q: If you did the multiple-tube technique, list one advantage and one disadvantage of each method of…

A: Water from various sources obtained for routine testing were analyzed by Swedish standard methods…

Q: How does an autoclave sterilize instruments? What is used to dry glassware and at the same time

A: Autoclaving is the best method for sterilizing glass instruments. Sterilizing means removal of all…

Q: Identify the principles of the lyophilization process and list down the precautions and limitations…

A: Lyophilization is the process of freeze-drying also called cryodesiccation. It involves…

Q: What are the different types of Microbial Control

A: Microbial control agents are the biotically-derived chemical compounds or secondary metabolites that…

Q: What is the motivation behind the use of stab slant and surface inoculation

A: Agar slants are prepared by adding the agar media to the test tube and allowing it to cool in a…

Q: Introduction for the stab slant and surface inoculation.

A: Slant culture one made on the outside of solidified medium in a tube which has been shifted to give…

Q: Why don’t health departments routinely test for pathogens instead of using a sewage indicator?

A: The most widely used sewage indicator organisms are fecal coliforms. This is because these microbes…

Q: Explain why it is important to wash the wells with PBS between the additions of each reagent?

A: ELISA is a enzyme linked immunosorbent eassay. It is a process to get the measurements of antigens…

Q: use these OD numbers to plot growth curve for E. coli K12– and estimate generation time for this…

A: The growth curve for E.coli K12 is plotted as follows:

Q: Why should you filter some liquids for sterilization, rather than autoclaving them?

A: To control microbial growth or contamination there are a number of aseptic techniques developed that…

Q: What microbe requires serum components to be added to the growth medium?

A: Introduction Microorganisms:- An organism that can be seen only through a microscope, They live in…

Q: 1. Differentiate between defined and undefined culture media. 2. Define alpha, beta, and…

A: The culture medium is a solid, semi-solid or liquid substrate that is rich in nutrients and other…

Q: explain methods for cleaning beans using flat bed screen and drum spray cleaner

A: Much of the mechanical cleaning procedures must be performed in the field for proper storage of the…

Q: Calculate the CFU/ml of a urine sample if 49 coli colonies were counted on NA when 50 microliters…

A: In both basic and applied research, microbiology is a highly significant area. Proteomics, genomics,…

Q: Why are aseptic techniques implemented during inoculation?

A: Aseptic means without contamination and Inoculation means transfer of a bacterial sample onto a…

Q: In the conduct of serial dilution, which of the following tools is NOT necessary?   a. Agar…

A:

Q: Which piece of equipment sterilizes hospital tools and devices and how does it sterilize things? Are…

A: Sterilizing pieces of equipment are very important in modern healthcare settings. It is necessary…

Q: You have retrieved your MHA (Mueller-Hinton agar) plate, your materials, and your E. coli culture.…

A: Answer : First step of the kirby-Bauer test :-

Q: How would you inoculate a plate to get a 1:10 dilution? A 1:100 dilution?

A: Inoculation is defined as the process of introduction of microorganisms into a culture for their…

Microbial Growth

The development of microbial growth is influenced by various natural conditions (both physical and synthetic) like pH, temperature, saltiness, gas focus (O2, CO2, and H2), nutrients, water action, and assimilation. Knowing the distinctive physical and chemical factors that influence the development and endurance of microbial growth in the climate will assist with reinforcing endeavors outfitted towards their control. These lines lessen or diminish their harmful impacts on humankind, creatures, and the climate.