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- Hand draw the expected gel result from the graphical data of three extracted RNA samples (in Figure 1a, 1b, & 1c) in Nanodrop spectrophotometer. The 3 graphical data should be in 1 agarose gel Provide complete gel label descriptions (ladder, lanes, etc.).mixed micron cuboidal shaped cells within the stronger paste (F127) and the physical/biochemical process that occurs during the gelationYou're trying to purify a protein with the aid of an ion exchange column. On the column, the protein is currently immobilized. Describe a modification you might make to the buffer that runs across the column to allow the protein to elute from it.
- Why do we use a stacking gel in SDS-PAGEThe images attached are the photos of bacteria in yoghurt under a misroscope. According to these images and your own knowledge, can you make a biological drawing of bacteria in yoghurt ( in a circle) and identify the types of bacteria accurately?option isoelectric focusinggel filtrationsalting outdifferential centrifugationEdman degradationion exchangedialysis
- What's the amount of protein if the measurement at A280 = 1.120, dilution factor is 100 and total volume of extract is 45Expose the gel to UV light. Explain why the GFP band does notfluoresce.Bacterial Growth lab using E. coli broth culture solution: Each 20-minute interval, absorbance of culture was recorded. When the curve showed plateaus was the absorbance for each culture proportional to the number of bacteria present in the tubes?
- In SDS page, the ________ proteins will move through the gel slowest and last, and in size exclusion chromatography, the _________ proteins will elute from the column first. smallest; largest largest; largest smallest; smallest largest; smallestThe essence of the electrophoresis method. The main components of the installation for carrying out electrophoresis. Application of this method to study the properties of various molecules. Electrophoretic mobility, what it depends on.Show the calculations required to make up 250mlof a stock solution of this chemical that will then be at the working concentration when 1 volume of this solution is added to 10 volumes of cell culture medium.