When doing automated sequencing, all 4 dideoxynucleotides are added to the same sequencing reaction, and run together in a single capillary gel. What's the difference - why can an automated sequencing reaction be done with all 4 dideoxynucleotides mixed together and all the resulting fragments run together, but not a conventional sequencing reaction?

When doing automated sequencing, all 4 dideoxynucleotides are added to the same sequencing reaction, and run together in a single capillary gel. What's the difference - why can an automated sequencing reaction be done with all 4 dideoxynucleotides mixed together and all the resulting fragments run together, but not a conventional sequencing reaction?

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter19: Genomes And Proteomes

Section: Chapter Questions

Problem 1ITD: Below is a sequence of 540 bases from a genome. What information would you use to find the...

See similar textbooks

Similar questions

Whether done manually or automated, DNA sequencing gels are always made of polyacrylamide rather than agarose. Why can't agarose be used for a sequencing gel, as it is for other DNA gel electrophoresis?
Download BLOSUM30 and BLOSUMB0 substitu- tion matrices and place them side by side on your computer screen. What are the differences between the two matrices? Why do you see these differences?
What special kind of nucleotide is used in the Sanger sequencing procedure?
How does what you see on the acrylamide gel reflect what is presented on a sequencing chromatograph?
What is the purpose of the dideoxyribonucleoside triphosphates in the dideoxy-sequencing reaction?
Why does the blood from the peripheral, tumor and breast samples all show bands of DNA that are 3000 bases and 1282 bases long?
If one DNA segment has the following base composition, 5'-CAGTTAGTCA-3', which of the following sequences is complementary? 5'-TGACTAACTG-3' 3'-TGACTAACTG-5' 3'-CAGTTAGTCA-5' 3'-TGACTAACTG-5'
Sanger sequencing originally used 4 lanes in gels. These lanes represented sequences of different lengths obtained by adding: All of the 4 dideoxynucleotides (ddATP; ddGTP; ddCTP; ddTTP), together with all of the 4 deoxynucleotides (dATP, dGTP, dCTP and dTTP), to all of the reaction vials All of the 4 dideoxynucleotides (ddATP; ddGTP; ddCTP; ddTTP) to the reaction vials; together with one of the 4 deoxynucleotides (dATP, dGTP, dCTP and dTTP), one for each lane, in each vial. One of the 4 dideoxynucleotides (ddATP; ddGTP; ddCTP; ddTTP) to the reaction vials; one for each lane, together with all of the 4 deoxynucleotides (dATP, dGTP, dCTP and dTTP) in each vial One of the 4 dideoxynucleotides (ddATP; ddGTP; ddCTP; ddTTP) to the reaction vials; one for each lane
In reversible terminator sequencing, how would the sequencing process be affected if the 3′-end-blocking group of each nucleotide were replaced with the 3′-H present in the dideoxynucleotides used in Sanger sequencing?
Consider the DNA segment with a sequence: 3'-TACGGTACGGGATTG-5'. If the given DNA sample was subjected to pyrosequencing, sketch the expected profile of the sequencing output. Assume that the sequential flooding of nucleotides follows the order G-C-A-T.
What are the consequences for a DNA sequencing reaction if the ratio of dideoxyribonucleoside triphosphates to deoxyribonucleoside triphosphates is increased? What happens if this ratio is decreased?
Why evenly spaced sequence in chromatogram is an indication of a good DNA chromatogram?