Why does DNA polymerase require a primase in order to begin DNA replication? O DNA replication requires a 3'-OH to act as nucleophile and it is provided by the RNA primer produced by primase
Q: Which type of DNA damage can be addressed by non-homologous end joining? O Pyrimidine dimers O…
A: Non homologous end joining is a repair pathway for double stranded breaks in DNA. It is called…
Q: Which DNA repair mechanism is described? In E. coli, MutH recognizes the correct template through…
A: DNA repair, or any of the several ways in which a cell maintains the integrity of its genetic code.…
Q: DNA ligase catalyzes the formation of a peptide bond without adding another nucleotide to the…
A: The DNA backbone is made up of deoxyribose sugar and phosphate groups. The phosphate group joins two…
Q: What is the role of the DNA Polymerase I 5' --> 3' exonuclease activity at a replication fork? O…
A: DNA polymerase I is an enzyme which has 3 functional activities which are : 5'-3' polymerase…
Q: The palm domain of a DNA polymerase O contains the catalytic site of the enzyme Ograbs the incoming…
A: The majority of organisms' genetic material, deoxy ribonucleic acid (DNA), contains nucleotide…
Q: During retrotransposițion of a short interspersednuclear element (SINE), nicking the target site DNA…
A: A complete genetic material present in an organism is referred to as a genome. Deoxyribonucleic acid…
Q: Which of the following does NOT play a role in DNA replication? O helicase promoter O ligase…
A: DNA replication: It is a process of making new strands of DNA with the help of several enzymes…
Q: Proof-reading during DNA replication refers to: * O removal of thymine dimers by by excision repair…
A: DNA polymerases are the enzymes that build DNA in cells. During DNA replication (copying), most DNA…
Q: In replicationbinds to the origin of replication cauning a short section of DNA to unwind. O DraA…
A: DNA replication is the biological process of copying the contents of DNA ie, forming two identical…
Q: The reaction in DNA replication catalyzed by DNA ligase isa) Addition of new nucleotides to the…
A: The term DNA stands for deoxyribonucleic acid. Deoxyribonucleic acid is the most important…
Q: DNA replication AND repair both finish with the action of Primase DNA polymerase l DNA polymerase II…
A: Primase- Primase is an enzyme that produces primers, which are short RNA sequences. Primase works by…
Q: correct chronological order of the initiation of DNA replication?
A: Initiation of DNA replication involves making the target DNA accessible to the enzymes and proteins…
Q: this 14. Synthesize a complementary strand of DNA for this strand:
A: DNA is a double helical structure. In DNA, strands are antiparallel , one strand is in 5’ to 3’…
Q: A deamination occurs on the cytosine residue in the following DNA sequence. This cytosine residue…
A: Deamination is one of the common forms of "hydrolytic DNA damage" in living organisms. Deamination…
Q: Why is telomerase not needed in prokaryotic DNA replication? O Prokaryotes have circular DNA O…
A: The telomerase is responsible for end replication in case of eukaryotic cells which perform RNA…
Q: Polymerases usually add only about 10 nucleotides toa DNA strand before dissociating. However,…
A: Nucleotides are the molecules composed of a sugar moiety, phosphate group, and a nitrogenous base.…
Q: Semi-conservative replication results in two double helix molecules, each with mixed old and new…
A: Semi-conservative replication occurs when two strands of DNA unzip, and a new strand is assembled…
Q: Polymerases usually add only about 10 nucleotides to a DNA strand before dissociating. However,…
A: The DNA replication is carried out by an enzyme called as DNA polymerase enzyme that can only add…
Q: During DNA replication, one of the new strands of DNA is synthesized continu while the other is…
A: DNA replication is a process in which DNA makes copies of itself with the help of the enzyme DNA…
Q: The palm domain of a DNA polymerase O contains the catalytic site of the enzyme CIC Site
A: Deoxy ribonucleic acid (DNA) is the genetic material of most organisms that carry coded genetic…
Q: he bases of one of the strands of DNA in a regionwhere DNA replication begins are shown at the endof…
A: Replication is the biological process in which a single copy of DNA is duplicated into two copies…
Q: Optimal DNA replication requires the coordinated effort of all the following EXCEPT: A single strand…
A: Single stranded binding proteins prevent single stranded DNA from exonuclease activity during…
Q: All known DNA polymerases catalyze synthesis only in the 5' → 3' direction. Nevertheless, during…
A: DNA replication is the process of making two identical copies of DNA. The process is carried by the…
Q: In E. coli, RecBCD complex has (select all correct answers) endonuclease activity а. O b. DNA…
A: RecBCD is a enzyme of e.coli which is required to repair thier DNA. RecBCD has many components and…
Q: A DNA strand contains a mismatched thymine. What type of DNA damage has occurred to cause this…
A: It is a multiple choice question.
Q: Describe how this type of DNA repair works
A: DNA damage is caused due to chemical addition or deletion of a base of DNA which create an abnormal…
Q: DNA Polymerase of E.coli has 3 enzymatic activities. One is a 5'- 3 exonuclease. 1. what is its…
A: Since you have asked multiple question, we will solve the first question for you. If you want any…
Q: The Escherichia coli chromosome is a circular DNA molecule and contains a single origin of…
A: Introduction : DNA replication in E.coli is a process through which daughter DNA synthesized from…
Q: Which of the following would be involved in the excision repair of a thymine dimer? O DNA polymerase…
A: DNA is the nucleic acids present in the organisms. DNA is the deoxy ribose nucleic acid in which…
Q: Replication of DNA requires a primer to initiate DNA synthesis because DNA polymerase can add new…
A: DNA replication is the process by which new DNA is produced from the old DNA in the…
Q: All of the following are involved in DNA replication excepta) polysome. b) gyrase. c)…
A: DNA is the genetic and hereditary material in all living organisms. When a cell divides, DNA is…
Q: What is the role of TOPOISOMERASES in DNA replication? In simple terms, so that I can understand
A: Topoisomerases (also known as DNA topoisomerases) are enzymes involved in the over- or underwinding…
Q: Primase has the important role of removing the RNA primers and filling the gaps with new DNA…
A: DNA replication There are number of processes necessary for the continuation of generation, growth…
Q: form a covalent linkage to both strands of the DNA helix at the same time, making a translent…
A: Replication: The process of replicating a double-stranded DNA molecule into two identical DNA…
Q: Escherichia coli's chromosome has a replication origin called OriC. Draw a schematic diagram to show…
A:
Q: DNA pol I O is a single subunit enzyme has a 5' to 3' exonuclease activity
A: DNA polymerase progressively adds deoxyribonucleotides to the 3'OH end of the newly synthesised…
Q: All of the following are critical factors for DNA replication of the leading strand? Check all that…
A: DNA replication is the process by which new DNA is synthesized from the old DNA by the…
Q: The DNA polymerase I uses its to remove the RNA primers during DNA replication in E. coli cells.…
A: DNA synthesis is a semiconservative process in both eukaryotes and prokaryotes. This means that each…
Q: During DNA replication, one of the new strands of DNA is synthesized continuously, while the other…
A: Introduction:- Each of the two parental DNA strands acts as a template for new DNA to be generated…
Q: Which of the following statements are true about DNA polymerase? Select all that apply. O On the…
A: Polymerases are enzymes that catalyze the synthesis of DNA or RNA polymerase whose sequence is…
Q: Which of the following DNA repair mechanisms involves the 3' to 5' exonuciease function of DNA…
A: DNA Polymerase is the enzyme that catalyzes the synthesis and replication of DNA. It plays a vital…
Q: DNA polymerase III has Options O 5 to 3' polymerase activity O 3'-5' exonuclease activity OBoth A…
A: The polymerase enzyme helps in building lengthy polymer or nucleic acid chains.
Q: DNA polymerase III in bacteria is responsible for initiating DNA replication by adding nucleotides O…
A: Introduction:- Replication is a process by which a cell duplicates it's genetic material. There are…
Q: During DNA replication, the function of RNA primers is to O serve as a binding site for DNA ligase…
A: Introduction : Primer is a short sequence that is synthesised by primase (a type of RNA polymerase)…
Q: DNA replication occurs O On both parental strands semi-conservatively In both directions From 5'-->…
A: Ans- All of the above.
Q: Several proteins involved in DNA repair are used in multiple pathways. Which one of the following is…
A:
Q: the tus ter complex can arrest the replication fork in one direction only. why is it necessary or…
A: Arrest of DNA replication is trigerred by the encounter of a replisome with a tus protein ter…
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- Replication involves a period of time during which DNA is particularly susceptible to the introduction of mutations. If nucleotides can be incorporated into DNA at a rate of 20 nucleotides/second and the human genome contains 3 billion nucleotides, how long will replication take? How is this time reduced so that replication can take place in a few hours?Sometimes DNA polymerase makes a mistake, and the wrong nucleotide is added to the growing DNA strand. Withregard to pyrimidines and purines, two general types of mistakes are possible. The addition of an incorrectpyrimidine instead of the correct pyrimidine (e. g. adding cytosine where thymine should be added) is called atransition. If a pyrimidine is incorrectly added to the growing strand instead of purine (e.g. adding cytosine when anadenine should be added), this type of mistake is called a transversion. If a transition or transversion is not detected by DNA polymerase, a mutation is created the permanently changes the DNA sequence. Though both types of mutations are rare, transition mutations are more frequent than transversion mutations. What are at least three explanations as to why this is the case?What statement(s) apply to both eukaryotic and prokaryotic replication? Select all that apply. a. There is a single origin of replication. b. Replication is discontinuous on the lagging strand and continuous on the leading strand. c. The sliding clamp is composed of PCNA. d. Telomeres are replicated by telomerase. e. Helicase unwinds the helix. f. Replication is continuous on the lagging strand and discontinuous on the leading strand.
- This is the original strand of DNA: ATG AAG TTT GGC TAA - what would represent a frameshift mutation due to deletion during replication ?DNA polymerase occasionally incorporates the wrong nucleotide during DNA replication. If left unrepaired, the base-pair mismatch that results will lead to mutation in the next replication. As part of a template strand, the incorporated wrong base will direct the incorporation of a base complementary to itself, so the bases on both strands of the DNA at that position will now be different from what they were before the mismatch event. The MER-minus strain of yeast does not have a functional mismatch excision repair system, but it has normal base excision repair and nucleotide excision repair systems. Which of the following statements is correct about differences in the mutation spectrum between MER-minus and wildtype yeast? More than one answer is correct. Options: More point mutations will arise in MER-minus yeast. Fewer point mutations will arise in MER-minus yeast as compared with wildtype. Of the total point mutations that…Ultraviolet light can cause covalent linkages between consecutive pyrimidine bases in DNA (up to 100 per second in a single cell in sunlight!). These bulky lesions (i.e. cyclobutane pyrimidine dimers and 6-4 photoproducts), which inhibit DNA and RNA polymerases, are mostly reversed by CPD photolyase when light >300 nm is available to power the reaction. In the dark, however, which DNA repair system is best able to correct these errors? a) non-homologous end-joining b) mismatch repairc) nucleotide-excision repaird) base-excision repair e) homology-directed repair
- All known DNA polymerases catalyze synthesis only in the 5' → 3' direction. Nevertheless, during semiconservative DNA replication in the cell, they are able to catalyze the synthesis of both daughter chains, which would appear to require synthesis in the 3' → 5' direction on one strand. Explain the process that occurs in the cell that allows for synthesis of both daughter chains by DNA polymeraseA deamination occurs on the cytosine residue in the following DNA sequence. This cytosine residue happens to be methylated on the 5-position of the aromatic ring. 5'-GCATGG-3'. (Note: the top strand is shown; this is the strand where the deamination occurs.) If the mutation is not repaired, and a round of DNA replication occurs, then the sequence of the newly- replicated complementary strand (i.e., the bottom strand) will be: A. 5'-CCATGC-3 B. 5'-CCATAC-3' C. 5'-CATACC-3' D. 5'-GTATGG-3' E. None of the aboveWhat result would Meselson and Stahl have obtained if the replication of DNA were conservative (i.e., the parental double helix stayed together)? Give the expected distribution of DNA molecules after 1.0 and 2.0 generations for conservative replication.
- The anti-viral drug Acyclovir is a nucleotide analog that is lacking the 3’ OH group which is required to form a 3’→5’ phosphodiester bond. This drug is ineffective against DNA polymerases with proofreading abilities, which is why human DNA polymerases are not targeted. Acyclovir can be used to treatsevere cases of Epstein-Barr viral (EBV) infection, but has little to no effect under non-severe infections. Based on this information, EBV will use ________ DNA polymerase during severe infections and __________ DNA polymerase during non-severe infections. Human; Human EBV; Human EBV; EBV Human; EBVDepurination of purine bases results in an apurinic site. Assume a single depurination event occurs in the GC base pair of the sequence below and is not repaired. Then, if two rounds of replication occur, which of the following DNA sequences will exist after two rounds of replication? Remember that when DNA polymerases encounter an apurinic site, most often an A is incorporated into the newly synthesized strand. Assume this is true for the sequence below. ...TACT... ...ATGA... Question 7 Select one or more: ...TAGT... ...ATCA... 1. ...TACT... ...ATGA... 2. ...TAAT... ...ATTA... 3. ...TAAT... ...AT_A... 4. ...TA_T... ...ATAA... 5. ...TATT... ...ATAA...Depurination of purine bases results in an apurinic site. Assume a single depurination event occurs in the GC base pair of the sequence below and is not repaired. Then, if two rounds of replication occur, which of the following DNA sequences will exist after two rounds of replication? Remember that when DNA polymerases encounter an apurinic site, most often an A is incorporated into the newly synthesized strand. Assume this is true for the sequence below. ...TACT... ...ATGA... Question 7 Select one or more: a) ...TAGT... ...ATCA... b) ...TACT... ...ATGA... c)...TAAT... ...ATTA... d) ...TAAT... ...AT_A... . e) ...TA_T... ...ATAA... f)...TATT... ...ATAA...
