DNA polymerase III in bacteria is responsible for initiating DNA replication by adding nucleotides O to the 5' end of an RNA primer on single-stranded templates without the need for an RNA primer O in a 3' to 5' direction in the place of the primer RNA after it is removed O to the 3' end of an RNA primer
Q: ЗА. This DNA after Bsal digestion, produces a DNA fragment with sticky ends as shown in the figure…
A: Introduction :- DNA strands are digested with the help of various restriction enzymes .Bsal is an…
Q: DNA polymerase can catalyze elongation in the 5' to 3' direction. The strand that is replicating…
A: Initiation of the replication takes place at the specific initiation site of the DNA double helix.…
Q: Each DNA sequence that can serve as an origin of replication is found to contain a binding site for…
A: On a chromosome or a plasmid, an origin of replication can be referred to as a stretch of DNA where…
Q: DNA synthesis has a very low error rate. One reason for this is that the DNA polymerase enzyme can…
A: DNA polymerase is the enzyme that does maximum of the work throughout DNA replication. It builds a…
Q: DNA polymerase IIl sometimes makes a mistake during replication and builds the wrong nucleotide into…
A: A mutation is a phenomenon in which the Deoxyribonucleic Acid (DNA) segments are inserted into or…
Q: During retrotransposițion of a short interspersednuclear element (SINE), nicking the target site DNA…
A: A complete genetic material present in an organism is referred to as a genome. Deoxyribonucleic acid…
Q: What is the importance of the 3' to5' exonucleasevactivity of DNA polymerase
A: Enzymes serve as biological catalysts that fasten the rate of reaction by lowering the activation…
Q: Which of the three Mut proteins isresponsible for ensuring that the mismatched basein the newly made…
A: Mismatch repair or MMR is the system that recognizes and repairs any misincorporated, deleted, and…
Q: Sometimes DNA polymerase makes a mistake, and the wrong nucleotide is added to the growing DNA…
A: Point mutation : It occurs as result of replacement of one nucleotide by other. point mutation bring…
Q: What is the function for telomerase, a reverse transcriptase? O to recognize the tip of an existing…
A: The production of new DNA from old DNA is known as DNA replication that occurs within the S-phase of…
Q: A deamination occurs on the cytosine residue in the following DNA sequence. This cytosine residue…
A: Deamination is one of the common forms of "hydrolytic DNA damage" in living organisms. Deamination…
Q: The restriction endonuclease NotI recognizes the octanucleotide sequence GCGGCCGC. Calculate the…
A: Restriction endonucleases are enzymes that cut DNA sequences at a specific site known as a…
Q: What is the following molecules not required for DNA synthesis during S- phase? * O RNA primer…
A: DNA replication is the process by which new DNA is synthesized from the old DNA. In case of…
Q: Polymerases usually add only about 10 nucleotides to a DNA strand before dissociating. However,…
A: The DNA replication is carried out by an enzyme called as DNA polymerase enzyme that can only add…
Q: 3 The restriction endonuclease PstI cuts DNA symmetrically on both strands at the СTGCAG sequence:…
A: Restriction endonucleases are the enzymes that are used to form cuts inside the DNA molecule. It is…
Q: Phosphorus is required to synthesize the deoxyribonucleoside triphosphates used in DNA replication.…
A: DNA replication is a semi-conservative process in which newly synthesized double helix DNA consists…
Q: One common feature of all DNA polymerases is that they a. synthesize DNA in the 3′-to-5′…
A: One common feature of all DNA polymerases is that they synthesize DNA in the 5'-3' direction.
Q: During high stress environments, it has been found that some bacteria activate a genetic mechanism…
A: The given enzymes are used in the replication process. It is a process wherein the genetic material,…
Q: There are various types of DNA-targeting drug, including DNA alkylating agents, DNA intercalators…
A: Mutations are DNA errors that occur during life. A mutation is defined as any alteration in the DNA…
Q: In E. coli, RecBCD complex has (select all correct answers) endonuclease activity а. O b. DNA…
A: RecBCD is a enzyme of e.coli which is required to repair thier DNA. RecBCD has many components and…
Q: The following sequence of nucleotides is found in a single-stranded DNA template:…
A: The process of formation of mRNA from DNA is called transcription.
Q: Which of the following statements is FALSE regarding the molecular mechanism for DNA polymerases?…
A: A DNA polymerase is the member of family of enzymes that catalyze the synthesis of DNA molecules…
Q: FIGURE 19.16 Direct repair of dam- aged bases in DNA. (a) The repair of thymine dimers by…
A: The DNA (deoxyribonucleic acid) is the hereditary unit. It is a double stranded structure of…
Q: If primase makes an error and incorporates an incorrect base in the nucleic acid primer, then why…
A: An incorrect base will not pair correctly with the template strand. Its greater structural…
Q: 1. The two sequences shown below are complementary to each other. T or F GTCGAC CAGCUG 2. Telomerase…
A: The image shows 10 statements. We have to determine whether the statement is True or False. The…
Q: Similar to prokaryotes, eukaryotes have a topoisomerase-ll-class enzyme called DNA gyrase, which can…
A: Topoisomerases are enzymes that catalyze the overwinding or underwinding of the DNA. This issue is…
Q: Which of the following reactions is required for proofreading during DNA replication by DNA…
A: DNA polymerase III is the primary enzyme complex which is involved in prokaryotic DNA replication.…
Q: Any RNA polymerase in any organism: OA Synthesizes RNA chains in the 3' to-5 direction O B. Binds…
A: A gene is a functioning heredity unit made up of DNA that provides instructions for the creation of…
Q: Okazaki fragments are a direct consequence of: O The lagging strand being more highly methylated…
A: DNA replication is the process well known as to define as the process by which a double-stranded DNA…
Q: ven though the eukaryotic genome is thousands of times larger than the prokaryotic genome, DNA…
A: Answer: EUKARYOTIC GENOME is more complex and larger than PROKARYOTIC GENOME beacuse prokaryotic…
Q: Mistakes made by DNA polymerase are corrected either by proofreading mechanisms during DNA…
A: The majority of the missteps during DNA replication are immediately amended by DNA polymerase which…
Q: Please help me with the orange question. My answer is leading strand will encounter lagging strand.…
A: DNA replication takes place in a bidirectional mode in which both strands are replicated…
Q: RNA primase is required for DNA replication a)on the lagging strand to ligate okazaki fragments.…
A: Deoxyribonucleic acid (DNA) stores the cell’s genetic information and is present in the nucleus of…
Q: Replication in eukaryotes begins at the Oric sequence. This particular sequence is characterized by…
A: Hi, Thanks For Your Question. Question 15 Answer : Correct Answer Is Option B. Reason For Correct…
Q: DNA pol I O is a single subunit enzyme has a 5' to 3' exonuclease activity
A: DNA polymerase progressively adds deoxyribonucleotides to the 3'OH end of the newly synthesised…
Q: Mutation of subunit epsilon (ɛ) of DNA polymerase II will result in an E. coli strain that has a…
A: Question -Mutation of subunit epsilon (ε ) of DNA polymerase lll will result in an E. coli strain…
Q: Which of the following statement(s) is/are false/incorrect? You may select multiple options, if…
A: Introduction :- A DNA polymerase is an enzyme that catalyzes the manufacture of DNA molecules from…
Q: From the diagram to the right of the trp repressor in its (i) approximate binding relationship to a…
A: Given Figure shown trp repressor protein bond to DNA double strand. Protein is mainly comprised of…
Q: It is essential that RNA primers at the ends of Okazakifragments be removed and replaced by DNA…
A: Introduction DNA replication is very crucial for the continuation of life as every new daughter…
Q: Why does DNA polymerase require a primase in order to begin DNA replication? O DNA replication…
A: DNA polymerase (DNAP) is defined as the type of enzyme responsible for making new copies of DNA.…
Q: The DNA polymerase I uses its to remove the RNA primers during DNA replication in E. coli cells.…
A: DNA synthesis is a semiconservative process in both eukaryotes and prokaryotes. This means that each…
Q: During DNA replication, one of the new strands of DNA is synthesized continuously, while the other…
A: Introduction:- Each of the two parental DNA strands acts as a template for new DNA to be generated…
Q: Select the statement(s) that accurately describe the function of DNA polymerase and the types of…
A: Mutation is a phenomenon that results in alteration of DNA sequences. This results in changes in the…
Q: A mutation in which of these proteins will lead to increased mutations in all daughter cells? A.…
A: The mutation is a heritable change that arises due to the alteration of the genomic sequence of an…
Q: The function of gamma (y) complex of DNA polymerase III is to Select one: O a. to unwind double…
A: DNA polymerase III falls in the category of an holoenzyme that has two core enzymes, a sliding clamp…
Q: Telomerase adds six deoxynucleotides to the end of a DNA strand using a built-in RNA template.…
A: The ends of eukaryotic chromosomes are called telomeres, and they are composed of repeats of a…
Q: The restriction endonuclease NotI recognizes the octanucleotide sequence GCGGCCGC. Calculate the…
A: restriction endonuclease is an enzyme that cuts DNA sequences at specific sites.
Q: During DNA replication, the function of RNA primers is to O serve as a binding site for DNA ligase…
A: Introduction : Primer is a short sequence that is synthesised by primase (a type of RNA polymerase)…
Q: Several proteins involved in DNA repair are used in multiple pathways. Which one of the following is…
A:
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- Chemical Mutagenesis of DNA Bases Show the nucleotide sequence changes that might arise in a dsDNA (coding strand segment GCTA) upon mutagenesis with (a) HNO2, (b) bromouracil, and (C) 2-aminopurine.Heteroduplex DNA Formation in Recombination From the information in Figures 28.17 and 28.18, diagram the recombinational event leading to the formation of a heteroduplex DNA region within a bacteriophage chromosome.From standpoint of replication and transcription, explain how RNA polymerase is allowed to incorporate the first nucleotide whereas DNA polymerase needs a primer. Explain how this difference impacts the process of replication and transcription.
- Shown below is a long template strand of DNA where lagging strand DNA synthesis is occurring. The short horizontal lines represent two Okazaki fragments that have already been made. In the context of the replication fork, select the letter(d–g) that indicates where primase will synthesize the next RNA primer. Explain why did you choose that location?The following sequence of nucleotides is found in a single-stranded DNA template:ATTGCCAGATCATCCCAATAGATAssume that RNA polymerase proceeds along this template from left to right a. Which end of the DNA template is 5′ and which end is 3′?b. Give the sequence and identify the 5′ and 3′ ends of the RNA transcribed from this templateThe purpose of DNA gyrase in replication is: to relieve positive supercoiling induced by unwinding the DNA during replication. to induce tighter coiling in supercoils. to remove the RNA primers from the lagging strand at each Okazaki fragment to start unwinding and separate the DNA to initiate replication to provide energy to remove a diphosphate fragment from each nucleoside triphosphate.
- DNA polymerase requires both a template, to be copied, and a primer, which provides a 3′ hydroxyl from which polymerase can extend. Yet this molecule supports DNA polymerase activity. Explain. pTGACACAGGTTTAGCCCATCGATGGG−OH Match the items in the left column to the appropriate blanks in the sentence on the right.A deamination occurs on the cytosine residue in the following DNA sequence. This cytosine residue happens to be methylated on the 5-position of the aromatic ring. 5'-GCATGG-3'. (Note: the top strand is shown; this is the strand where the deamination occurs.) If the mutation is not repaired, and a round of DNA replication occurs, then the sequence of the newly- replicated complementary strand (i.e., the bottom strand) will be: A. 5'-CCATGC-3 B. 5'-CCATAC-3' C. 5'-CATACC-3' D. 5'-GTATGG-3' E. None of the aboveThe beta subunits of E.coli DNA polymerase III are responsible for its _______. A. ribosome assembly B. 5’→ 3’ polymerase C. 3’ → 5’ exonuclease and proof-reading D. sliding clamp
- The sequence of a region of interest in a DNA template strand is3′–ATACGACTAGTCGGGACCATATC–5′. If the primer in a dideoxysequencing experiment anneals just to the left of this sequence, drawthe sequencing ladder that will be obtained.During eukaryotic DNA replication, _________ synthesizes short RNA primers. The primers provide a 3'-OH group to which DNA nucleotides are be added by DNA polymerase. DNA gyrase (topoisomerase) DNA helicase DNA ligase primaseTelomerase adds six deoxynucleotides to the end of a DNA strand using a built-in RNA template. Explain why the templating region of the RNA includes one and a half repeats of the telomeric sequence (∼9 nucleotides).