Will a HPLC-UV method be more advantageous than a UV/Vis spectroscopic method for cefuroxime? Explain answer.

Q: How can l exploit the mobile phase composition to optimize my HPLC separation?

A: The technique which is commonly used in laboratory for the separation of components of mixture is…

Q: Focusing Lenses Radiation Source Wavelength Selector Detector Atomizer Signal Processor Amplifier…

A: 5) The capillary column are more efficient than packed columns in GC.

Q: If the TLC plates had been developed in a more polar solvent, would retention factors on the whole…

A:

Q: An ideal RF value for a compound on a developed TLC plate is 0.30. How could you adjust the TLC…

A:

Q: Why is it more difficult to optimize k' and α in HPLC than in GC hand

A:

Q: Dye 1 Dye 2 Run 1 Run 2 Run 3 Run 1 Run 2 Run 3 Start of Band (mL} 1.1 1.1 1.2 2.2 2.2 2.3 End of…

A: Retention Volume : It is the volume of the mobile phase which is required to elute out a particular…

Q: The following data were obtained by GC on a 40 cm packed column. ( VM: 1.28 mL, Vs: 0.65 mL)…

A: Solution:

Q: Justify following statements.  (a) Paper chromatography is a type of partition chromatography. (b)…

A: a) Partition chromatography is based on differential partitioning of components of a sample mixture…

Q: How to improve the sensitivity and selectivity of AFS for Hg detection? Please explain.

A: If we increase the intensity of excitation source in AFS ,  high sensitivity will be…

Q: The image below shows van Deemter curves for five different HPLC columns used under the same…

A:

Q: Relative Standard Deviation of UV Vis, please explain.

A: A question based on RSD, which is to be accomplished.

Q: The TLC shown above, similar to the silica (SiO₂) TLC plates you used in lab, w developed in a…

A: For the better understanding you need to go through the following concepts first.

Q: What safety precaution are needed when working with  a.) centrifuge  b.) Inductively Coupled Plasma…

A: a) safety precaution are needed when working with Centrifuge: Safety cups have to be used. The…

Q: föllowing analysis of a compounds (A-D) by were obtaine HPLC: Peak Retention time (min) Width of…

A: The full form of HPLC is high performance liquid chromatography. It is used to identify or separate…

Q: Will a HPLC-UV method be more advantageous than a UV/Vis spectroscopic method for cefuroxime?…

A: Please find your solution below :  HPLC-UV method is more advantageous than a UV/Vis spectroscopic…

Q: Differentiate between isocratic elution and gradient elution. Why is gradient elution sometimes a…

A:

Q: In a thin layer chromatography experiment, a plate of length 8.67 cm was used and a horizontal line…

A: Retention factor (Rf) is defined as the ratio of the distance travelled by the compound (dc) to the…

Q: I need help darwing a TLC plate  and figuring out the Rf values is the retention factor values We…

A: The crude mixture is the component that is obtained directly from the reaction after its completion.…

Q: ) In What order Ufrom highest Rf tolanest Rf) wonld you edpeet to fina 3 compardy Shopn below on a…

A:

Q: Two components in an HPLC separation have retention times that differ by 22 s. The first peak elutes…

A: Soln

Q: An LC separation specifies that the mobile phase be 25% tetrahydrofuran (THF) in water.…

A:

Q: Briefly discuss the importance and principles of non-contact laser profilometry.

A: The non-contact laser profilometry is based on the principle of optical light interference to scan…

Q: Is standardization against primary standard required for controlled potential coulometry ?

A: Controlled potential coulometry, as the name suggests, is a coulometry method that measures the…

Q: A sample that contained two components was separated on a column. The column had a length of 15.0…

A: Given: length of column = 15 cm initial resolution = 1.2 required resolution = 2.0 we have to find…

Q: 1. Virtual Lab Questic particular waveleng absorptivity? What same conditions?

A: Absorbance = -Log [Transmittance]   Transmittance = 66 % = 66100 = 0.66   Absorbance = -Log0.66 =…

Q: Sort the following molecules in order of increasing Rf value assuming a normal-phase silica TLC…

A: Here, TLC plate was used with a 10 : 1 hexane:ethyl acetate solvent system which is non-polar.…

Q: What happen  to the separation raid (RS) in HPLC if I change the mobile phase from methanol/water…

A: Given: We have to tell the effect of polarity on the separation rate in HPLC.

Q: Liquid column chromatography on alumina would separate p-nitrotoluene and p-nitroaniline O A. with…

A:

Q: why is an electro thermal atomizer more sensitive than flame atomizer?

A: The oldest technique used in Atomic absorption spectroscopy is the Flame atomizer.

Q: Why are the application of TGA more limited than those for DSC?

A: TGA is one of the thermogravimetric techniques. In this process changes in chemical properties and…

Q: which Characterization techniques and instrument is usually used to analyse the particle size…

A: Analytical chemistry is branch of chemistry that do the separation, identification, and the…

Q: 5. Using the diagram answer the questions below: Increasing time Sample appled to top of column…

A: To answer question related to chromatography:

Q: Justify following statements.  (a) Paper chromatography is a type of partition chromatography. (b)…

A: Since you have posted a question with multiple subparts, we will solve first three subparts for you.…

Q: What four values are needed to calibrate a CO2 sensor (IRGA)? B. Briefly describe how to obtain…

A: These sensors depends on the IR light source and detects the quantity of CO2 molecules. Calibration…

Q: If a plot between absorption and concentration was not linear and a plot between absorbance and bath…

A: We know that absorbance is given by the formula, A = ∈ c l where A = absorbancec = concentrationl=…

Q: In chromatographic separations the components in a mixture to separate into zones a O all of the…

A: Chromatography is a separation and identification technique of components of a given mixture.

Q: Two students A, B did the Flame photometric analysis by different atomization methods in which the…

A: Flame Atomic Absorption Spectroscopy (FAAS), commonly called flame spectroscopy is a branch of…

Q: The peak parameters for your internal standard, evaluated using a capillary column with a length of…

A: HETP is defined as unit of coloumn length sufficient to bring solute in the mobile phase. It is a…

Q: Compare gradient and isocratic mode in HPLC.

A: Gradient and isocratic mode in HPLC are the two different modes of operating condition in HPLC.

Q: Calculate the Rf value of the Blue spot on the TLC Plate below. (answer must have * 2 decimal…

A: Calculate Rf value of the blue spot on TLC.  From the figure,  Distance travelled by the solvent…

Q: In HPLC, fill in the blanks with the choices below: - chromatogram retention time void volume…

A: To fill in the blanks for HPLC:

Q: In TLC, point of origin refers to O The bhottom edge of the TLC plnto.

A: Question 1: Option 1 is the correct answer. The point of origin was sketched very lightly with…

Q: Please describe possible sources of error in HPLC experiments - systematic and random and how they…

A: HPLC(High performance liquid chromatography) Errors in HPLC can be systematic and random. Systematic…

Q: What are the disadvantages of using a non- ideal linear velocity on the chromatographic separation…

A:

Q: Sort the following molecules in order of increasing Rf value assuming a normal-phase silica TLC…

A: In thin layer chromatography is a technique to separate different components in a mixture based on…

Q: According to given TLC plate below which of the following statement(s) is/ are false ? |- Rf value…

A: It is given a TLC plate in which the solvent travels a distance of 0.75 (approximately), and…

Q: Can you think of a situation in which a UV spectrophotometer would be more useful visible light…

A: Interpretation -  To explain that a situation in which a UV spectrophotometer would be more useful…

Q: The pure samples, R & S-Carvone and will undergo which analyses below (choose all that are…

A: Since you have asked multiple question, we will solve the first question for you. If you want any…