According to given TLC plate below which of the following statement(s) is/ are false ? |- Rf value of component A is 0.47. l- The stationary phase in TLC is solid supported silica or Alumina. I- Less retained substance is B.
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- 1. Define sparging. What is the significance of this step in an HPLC analysis? 2. What are the two types of pre-column? What is the purpose of each type? 3. What are the two types of packing used in HPLC column? Describe each type. Please answer numbers 1-3. Need asapWhy are some of the detectors listed unsuitable for HPLC? Which of the GC detectors ) in the table below ) are suitable for HPLC?Which of the following characteristics is/are the advantage(s) of HPLC over GC? I. Only small amount of sample is required I. Can accommodate nonvolatile samples Ill. Highly selective CHOICES: O l only O ll only O Ill only O ll and Ill only
- What is a guard column and why is it used in HPLC but not in GC systems?If a column was run on the sample shown in lane 3, what would be the first compound to elute off of the column? (assume the same solvent and stationary phase are used) Which of the lanes on this TLC plate show pure compounds? Select all that apply.On the blank TLC provided to the right, please draw where you would expect to see the spots for B-carotene (lane 1), compound A (lane 2) and compound B (lane 3). This is SiO2 plate and the appropriate developing solvent is used.
- What will be the result of applying too much compound to a TLC plate?An ideal RF value for a compound on a developed TLC plate is 0.30. How could you adjust the TLC developing conditions to obtain an RF of 0.30 if it's too low or too high (Hint: Think about chromatography phases).A TLC plate showed two spots A and B with Rf 0.20 and 0.30, respectively. Sketch a TLC chromatogram with the above Rf values. (Hint: Start with a 10-cm TLC plate and allow 1 cm each for the start and end solvent fronts.) Determine the distances travelled from the start of the solvent front. ___?____ A ____?___ B Determine the distances left to the end of the solvent front. ___?____ A ____?___ B
- Explain why it is needed to filter the solvent before introducing it to the HPLC systemThin‑layer chromatography (TLC) can be used as a preliminary identification method for many amphetamine‑type stimulants (ATS). Using a 0.25 mm thick silica gel stationary phase and a 98.5:1.5 methanol:concentrated ammonia mobile phase, a spot was observed to have traveled 3.52 cm when the solvent front traveled 8.81 cm. What is the retardation factor (?F)for the compound that composes this spot?Draw a skitch for GC and HPLC instruments showing all parts