There are several ways to transform the Michaelis-Menten equation so as to plot data and derive kinetic parameters, each with different advantages depending on the data set being analyzed. One transformation of the Michaelis-Menten equation is the Lineweaver-Burk, or double-reciprocal, equation. Multiplying both sides of the Lineweaver-Burk equation by Vmax and rearranging gives the Eadie Hofstee equation. Vo Vo = -(Km) + Vmax [S] Vo Consider the plot of Vo versus for an enzyme-catalyzed [S] reaction. The control reactions (blue curve) did not contain any inhibitor. V₁ curve B curve C curve A V A Slope [S] Which of the other curves (A, B, or C) depicts this enzyme's activity in the presence of a competitive inhibitor? mask
There are several ways to transform the Michaelis-Menten equation so as to plot data and derive kinetic parameters, each with different advantages depending on the data set being analyzed. One transformation of the Michaelis-Menten equation is the Lineweaver-Burk, or double-reciprocal, equation. Multiplying both sides of the Lineweaver-Burk equation by Vmax and rearranging gives the Eadie Hofstee equation. Vo Vo = -(Km) + Vmax [S] Vo Consider the plot of Vo versus for an enzyme-catalyzed [S] reaction. The control reactions (blue curve) did not contain any inhibitor. V₁ curve B curve C curve A V A Slope [S] Which of the other curves (A, B, or C) depicts this enzyme's activity in the presence of a competitive inhibitor? mask
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Please answer the last question
Calculate the standard error of regression (SER) for the Lineweaver–Burk slope and the Eadie–Hofstee slope to compare the precision of each.
Eadie–Hofstee SER:
it is not 0.128, 0.028, 0.136, or 0.1362.
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