Wobble hypothesis indicates that: a. fatty acids are degraded 2 carbons at a time. O b. 16S-ribosomal RNA recognizes Shine-Dalgarno sequence of the mRNA and thus initiates protein synthesis. C. DNA replication is semi-conservative. d. the 5'base of anticodons may recognize 1, 2 or 3 bases in the codons and thus only 31 different tRNA molecules can recognize 61 codons. Oe. DNA replication proceeds bidirectionally.
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- 4a) Write out the protein sequence (the amino acids, in order) encoded by the mRNA sequence: 5'AUGCGACCUAGCUAUGGA3' b) How many different mRNA sequences could code for the protein sequence you determined in 4a? Explain how you came up with your answer (don't write out all the possible mRNA sequences - just explain your logic). Can you help with 4a and sub question bRNA can function: please explain your answer a.A) as a non-permanent template to synthesize protein b.B) to recruit the correct amino acid during translation c.C) as part of ribosomes to synthesize protein d.D) to help initiate DNA synthesis during replication e.Only A and C Only A, B and C A, B, C and DRNA in ribosome catalyzes formation of peptide bonds in all organisms. This supports the hypothesis that______. a. RNA can hold more information than DNA b. RNA is more stable than DNA c an RNA world existed prior to the rise of DNA d. proteins evolved before RNA
- Check your understanding about the following statements:I. Messenger RNA is a single-stranded molecule that carries the genetic information from DNA to the ribosome. II. Transfer RNAs convert the genetic information into defined amino acid sequences a. The first statement is true. The second statement is false. b. The first statement is false. The second statement is true. c. Both statements are true. d. Both statements are false.Evaluate the following statements. Which one statement is false? a. The active form of prokaryotic RNA polymerase is a haloenzyme. b. Anti-codons are a nucleotide triplet in transfer RNA that are complementary and antiparallel to the codons of messenger RNA c. During translation, the terminal carboxyl group of an existing peptide will form a peptide bond with the next amino acid in sequence. d. The amino acid R-group is necessary for the formation of tertiary and quaternary structures through covalent bonds, ionic bonds, hydrogen bonds, and hydrophobic interactions. e. The sigma factors of prokaryotes are responsible for binding a DNA promotor sequence so translation can begin. f. The terminal carboxyl and terminal amino groups of amino acids within a peptide are necessary for the formation of secondary structures through hydrogen bonds. g. A restriction enzyme can target supercoiled, relaxed, or linearized plasmid DNA. h.…Now try this example yourself. Fill in the complementary mRNA sequence to the DNA sequence listed below: DNA sequence = C A T G C A C C G T T A C G A RNA sequence =
- Which would be worse for an organism? Briefly explain your answer. B. The spliceosome can no longer form OR a particular gene has a mutation that removes the endonuclease site used in the addition of the polyA tailWhen would a ribosome bind to a promoter sequence? A) It wouldn't. A promoter is a DNA sequence, and ribosomes don't bind to DNA. B) When the ribosome needs to transcribe the gene that that promoter controls. C) When the ribosome is translating the DNA sequence of a gene. D) When there is a start codon (AUG) in the promoter sequence. .(a) Write the sequence of the mRNA molecule synthesized from a DNA template strand having the following sequence:5'–ATCGTACCGTTA–3' (b) What amino acid sequence is encoded by the following base sequence of an mRNA molecule? Assume that the reading frame starts at the 5’ end.5'–UUGCCUAGUGAUUGGAUG–3! (c) What is the sequence of the polypeptide formed on addition of poly(UUAC) to a cell-free proteinsynthesizing system?
- BIOLOGY ACTIVITY -Gene Mutations and Proteins Objective: To demonstrate how gene mutations affect the production of proteins? Procedure: Use the following base sequence of one strand of an imaginary DNA molecule: AATTGAACACATGCGCCC. 2. Write the base sequence for an mRNA strand that would be transcribed from the given DNA sequence. Place your results in the table below. Use your codon table provided below to determine the sequence of amino acids in the resulting protein fragment. Place your results in the table below. If the fifth base in the original DNA strand were changed from G to C, how would this affect the resulting protein fragment? Write the new protein fragment in the table below. If G were added to the original DNA strand after the third base, what would the resulting mRNA look like? How would this addition affect the protein? Show your results in the table below. Data: mRNA from Step 2 Protein Sequence from Step 3 Protein Sequence from Step…The diagram below shows the result of a hybridization experiment between a eukaryotic mRNA and the template strand of its gene. Which of the following statements is TRUE? a. This gene contains four exons. b. The gene’s exons are visible as unpaired DNA loops protruding from the DNA/RNA hybrid. c. The 5’ end of the RNA strand is on the right side of the drawing. d. A hybrid between a prokaryotic mRNA and its gene would result in a similar image. e. the poly-A tail of the mRNA hybridizes with a poly-T stretch in the DNA.1) Where in the heck did Class I transposons originate? a DNA mutations. b Bacteria. c Prophages. d Retroviruses. 2) What do you think about humans only having about 22,500 genes but we contain about 100,000 proteins?! a The production of quaternary shape in proteins can contribute to protein variation. b That's the work of the spliceosome! c Post-translation modifications in the Golgi Apparatus are responsible for some of that. d All the answers are correct.