You obtain an E coli mutant with a defect in the pathway that emakes histidine, an amino acid. Tell me whether or not it would grow on each of the following plates Al Enriched or complete media B) enriched or complete media plus histidine OMinimal or defined media DI Minimal or defined media plus histidine
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- E. coli can grow at a higher temperature in a complex mediumthan in a defined medium. Why?why is Lysozyme treatment useful in bacterial cell lysis when protien isolation is happening.If a culture of E. coli you are tracking grows from 5 x 103 cells/ml to 9 x 107 cells per ml in 4 hours, what is the approximate generation time of your E. coli? how can i slove this step by step a. About 14 minutesb. About 17 minutesc. About 20 minutesd. About 23 minutese. About 30 minutes
- Define the following terms:a. proenzymeb. positive cooperativityc. negative cooperativityd. zymogene. free radicalYou perform the Methyl-Red(MR)& Voges-Proskauer(VP)test and gather the followingresults: (Uploaded picture). VP test result is on the left, MR test result is on the right. A) Please interpret these results. B) Your fellow researcher and yourself are sure your bacterial sample is either E. coli or P. vulargis. Basedupon the work we have done in lab which methods will help you determine which bacteria it is? Please explain why, the results you would want to observe and provide analysis of those results.in isolating ribosomes from a yield sample, describe the ideal type of centrifugation for this separation technique based on the following:*Main type of centrifuge *Subtype centrifuge * Speed range *Operational temperature range*Type of Centrifugation
- If one of your unknown bacteria ferments lactose, you CANNOT assume that the other unknown will also ferment lactose. If one of your unknown bacteria ferments lactose, you CANNOT assume that the other unknown will also ferment lactose. True FalseIn the Messelsohn-Stahl experiment, there should be ______ band(s) in the centrifugation tube after two generations of bacterial growth in normal nitrogen. Group of answer choices None of these One light and one medium density Two light and heavy Three light bands One heavyA researcher is planning to plant antibiotic-resistant Escherichia coli pure bacteria sample in antibiotic (ampicillin) solid media in his experiment. Describe each of the sterilization methods that this researcher should use while preparing this solid medium with antibiotics and in cultivation of the bacteria on the solid medium, indicating the stages at which it should be used and giving the mechanism of action.
- Your animal cell culture laboratory suddenly suffered from a bacterial contamination. Several components like vitamins, yeast hydrolysate, and fetal calf serum are temperature sensitive and must be filtered through a bacteriological filter to add to the autoclaved animal cell culture media. After careful considerations, you found that there are no issues with the autoclaving, but there is something wrong with the filter sterilization process, and changing new batches of filter did not remove the bacterial contamination. Which of the following rationale best describes the probable cause? Group of answer choices A. The media is probably contaminated with E. coli, which passed through the 0.2-micron bacteriological filter. B. The media is probably contaminated with Mycoplasma, due to the lack of cell walls they are the only bacteria that can pass through the 0.2-micron bacteriological filter. C. All of the other answer choices could be correct D. The media is probably…The following results were obtained from a disk diffusion test for a strain of Escherichia coli. Chemotherapeutic Zone of inhibition (mm) A 7 B 10 C 18 D 3 Which drug above (A, B, C, or D) is most effective against this E. coli strain? ***Which drug above (A, B, C, or D) is most effective against Staphylococcus aureus? Explain your answer.For E-coli -Describe the Gram staining procedure -Describe how catalase test would have been done (if this test was used)- -Name all media that should have been inoculated to ID your unknown and why