EBK LABORATORY EXPERIMENTS IN MICROBIOL
11th Edition
ISBN: 9780134024431
Author: CASE
Publisher: PEARSON CUSTOM PUB.(CONSIGNMENT)
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Chapter 1, Problem 1CT
Summary Introduction
To determine:
The number of cells of Bacillus and yeast can be observed under a field of 2mm diameter.
Introduction:
Microscopy is a technique of observing an object under microscope that is not observable with naked eyes. The types of microscopy include, scanning probe microscopy, electron microscopy and optical microscopy. Optical microscopy uses light for illuminating and lenses to magnify the sample.
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If the diameter at the largest point of a cell covers, 20% of the field of view when observed at 400x magnification what would be the approximate diameter of the cell in micrometers??
A) The size of a cell seen under a microscope is 3mm Using a total magnification of 40x. What is the actual cell size and decide if the cell is prokaryote or eukaryote based on its size?
In the cell wall staining experiment of Bacillus megaterium, what color can you find in
a) cell wall and why?
b) cytoplasm and why?
Chapter 1 Solutions
EBK LABORATORY EXPERIMENTS IN MICROBIOL
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- Measure the length of Cell X using the ruler in Microsoft Word. Assuming the actual length to be 3 um, calculate the magnification. Show your complete solution. Based on Figure 1, what internal organization can be distinguished in cell X? Why? Based in Figure 1, can you see a limiting membrane? Can you deduce its presence? Why or why not? Figure 2 is an electron micrograph of the same type of bacterium as shown in Figure 1. The picture has been obtained by cutting a very thin section of the bacterial cell along its longitudinal axis. Measure the total length of the cell, and assuming the actual length to be 2.1 um, calculate the magnification. With reference with Figure 2, what are the major differences between the inclusions found in Figure 1 and the way they appear in Figure 2? What other structural features can be resolved?arrow_forwardWhich of the following would be best observed for a bacterial cell using phase-contrast microscopy? a. Motility of cells b. Bacterial nucleoid c. Cell wall d. Glycocalyxarrow_forwardWhat is the size in µm of a cell that is 135mm wide in my photo? The scale bar in the photo is 2.8cm long and represents 8µm.arrow_forward
- Why do we use sucrose solution when we observe plant cells? What explanation please..arrow_forwardLet’s say you wanted to look at cells of Bacillus cereus, which are rod-shaped cells that are about 4 µm long. If you were observing B. cereus with a microscope using the high-power lens, how big would the cells appear to be when you look at them?arrow_forwardWhy does an electron microscope have a higherresolution, or greater resolving power, than a lightmicroscope?arrow_forward
- Which electron or light microscopy technique would you use for the following? (also explain) For visualizing the fine structure of cellular organelles and cell architecture in the cell interior using very thin cross-sections made in Epon. To obtain sequential images of a macromolecule in order to study its activity in real-time. To obtain an extremely clear three-dimensional image of the nucleus of a single cell. What is the magnification of the image in a microscope using a 20x ocular and a 100x objective?arrow_forwardWhich of the following microscopes typically requires the use of vital dyes (like methylene blue) to visualize large subcellular structures in a living cell (like the nucleolus or the mitochondrion)? the scanning/tunneling electron microscope (STEM), with good resolution up to about 100,000,000x the transmission electron microscope (TEM), with good resolution up to about 100,000x the scanning electron microscope (SEM), with good resolution up to about 1,000,000x the compound light microscope, with good resolution up to about 1,500x all of the above microscopes would be equally useful in visualizing the interior of organellesarrow_forwardUsing an appropriate lens and oil immersion, what would be a microscope's maximum resolution (in micrometres) that could use a wavelength of 350 nm (assuming that such a device exists)? What would be the surface-to-volume ratio for the coccus-shaped bacterium twice the size of the maximum resolution you calculated? Why is the surface-to-volume ratio an important biological factor. Explain. Show all of your calculations.arrow_forward
- Explain the difference (in principle) between a light microscope and a transmission electron microscopearrow_forwardif you visualize the cytoskeleton of a cell that is expanding in one direction, you typically observed a strong orientation of the cytoskeleton. Please answer the following three questions. a. Would the cytoskeleton be oriented parallel or perpendicular to the direction of cell expansion? b. Would the cellulose fibers in the cell wall be parallel or perpendicular to the cytoskeleton? c. Explain why cytoskeleton, cellulose fibers, and direction of cell expansion have the relationship mentioned in a and b?arrow_forwardGive the functions and magnifications of transmission electron microscope and scanning transmission electronarrow_forward
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