(a)
To determine: The electron donor and an electron acceptor in the given system along with the most likely pathway of electron transport in a system where mitochondria were incubated with β-hydroxybutyrate, oxidized cytochrome c, ADP, Pi, and cyanide.
Introduction: Electron transport system (ETS) is comprised of a number of complexes that carry electron from its donors to its acceptors. ETS joins this transfer of electrons with the transfer of H+ ions across the mitochondrial membranes. The whole process of ETS drives the mechanism of ATP synthesis.
(b)
To determine: The number of moles of ATP that would be formed per mole of β-hydroxybutyrate oxidized, and write a balanced equation for the reaction that occurs in the given system where mitochondria were incubated with β-hydroxybutyrate, oxidized cytochrome c, ADP, Pi, and cyanide.
Introduction: Electron transport system (ETS) is comprised of a number of complexes that carry electron from its donors to its acceptors. ETS joins this transfer of electrons with the transfer of H+ ions across the mitochondrial membranes. The whole process of ETS drives the mechanism of ATP synthesis.
(c)
To explain: The purpose of adding cyanide to the system and the possible results if cyanide was not added in the reaction system where mitochondria were incubated with β-hydroxybutyrate, oxidized cytochrome c, ADP, Pi, and cyanide.
Introduction: Electron transport system (ETS) is comprised of a number of complexes that carry electron from its donors to its acceptors. ETS joins this transfer of electrons with the transfer of H+ ions across the mitochondrial membranes. The whole process of ETS drives the mechanism of ATP synthesis.
(d)
To explain: Whether the enzymes of the citric acid cycle would be active in the assay where mitochondria were incubated with β-hydroxybutyrate, oxidized cytochrome c, ADP, Pi, and cyanide, giving the reason for the same.
Introduction: Electron transport system (ETS) is comprised of a number of complexes that carry electron from its donors to its acceptors. ETS joins this transfer of electrons with the transfer of H+ ions across the mitochondrial membranes. The whole process of ETS drives the mechanism of ATP synthesis.
(e)
To explain: The importance of β-ketobutyrate not being
Introduction: Electron transport system (ETS) is comprised of a number of complexes that carry electron from its donors to its acceptors. ETS joins this transfer of electrons with the transfer of H+ ions across the mitochondrial membranes. The whole process of ETS drives the mechanism of ATP synthesis.
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- BiochemistryBiochemistryISBN:9781305577206Author:Reginald H. Garrett, Charles M. GrishamPublisher:Cengage Learning