Microbiology Fundamentals: A Clinical Approach
3rd Edition
ISBN: 9781259709227
Author: Marjorie Kelly Cowan Professor, Heidi Smith
Publisher: McGraw-Hill Education
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Chapter 10, Problem 12Q
Summary Introduction
To determine:
The reason for provision of permanent solution to antibiotic resistance by CRISPR technique, unlike other temporary fixes.
Introduction:
Many antibiotics are natural products of fungi and bacteria; therefore, microbes have developed a survival strategy involving resistance to these antibiotics. As a result, it is very crucial to take up novel strategies to produce antimicrobials that can replace, and improve the existing antimicrobials that are losing their efficacy. One such strategy is the CRISPR technique.
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DNA can be separated based on fragment size using ________.
a.
restriction enzymes
b.
gel electrophoresis
c.
PCR
which sample shows the plasmid is fully digested
Restriction Enzymes with LongerRecognition Sites Produce Larger _______ Fragments
Chapter 10 Solutions
Microbiology Fundamentals: A Clinical Approach
Ch. 10.1 - State the main goal of antimicrobial treatment.Ch. 10.1 - Identify the sources for the most commonly used...Ch. 10.1 - Describe two methods for testing antimicrobial...Ch. 10.1 - Prob. 4AYPCh. 10.1 - NCLEX PREP 1. An RN is caring for a 26-year-old...Ch. 10.2 - Explain the concept of selective toxicity.Ch. 10.2 - List the five major targets of antimicrobial...Ch. 10.2 - Prob. 7AYPCh. 10.2 - Distinguish between broad-spectrum and...Ch. 10.2 - Prob. 9AYP
Ch. 10.2 - Explain the mode of action of penicillinases and...Ch. 10.2 - Identify two antimicrobials that act by inhibiting...Ch. 10.2 - Prob. 12AYPCh. 10.2 - Identify one example of a fluoroquinolone.Ch. 10.2 - Describe the mode of action of drugs that target...Ch. 10.2 - Prob. 15AYPCh. 10.2 - Prob. 16AYPCh. 10.2 - Explain why antiprotozoal and antihelminthic drugs...Ch. 10.2 - List the three major targets of action of...Ch. 10.2 - Prob. 2NPCh. 10.3 - Discuss two main ways that microbes acquire...Ch. 10.3 - List five cellular or structural mechanisms that...Ch. 10.3 - Prob. 21AYPCh. 10.3 - Prob. 3NPCh. 10.3 - Prob. 1MMCh. 10.4 - Distinguish between drug toxicity and allergic...Ch. 10.4 - Prob. 23AYPCh. 10.4 - Prob. 4NPCh. 10.4 - Prob. 5NPCh. 10 - Microbial resistance to drugs is acquired through...Ch. 10 - Prob. 2QCh. 10 - Prob. 3QCh. 10 - Prob. 4QCh. 10 - Why does the penicillin group of antibiotics have...Ch. 10 - Prob. 6QCh. 10 - Prob. 7QCh. 10 - Conduct research to find out why drugs blocking...Ch. 10 - Prob. 9QCh. 10 - Prob. 10QCh. 10 - You take a sample from a growth-free portion of...Ch. 10 - Prob. 12QCh. 10 - Treating malarial infections is theoretically...Ch. 10 - Can you think of a situation in which it would be...Ch. 10 - Prob. 15QCh. 10 - Prob. 16QCh. 10 - Prob. 17QCh. 10 - Prob. 18QCh. 10 - An antimicrobial drug with a _______ therapeutic...Ch. 10 - Prob. 20QCh. 10 - Prob. 21QCh. 10 - Prob. 1VC
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Based on the image below, answer the following questions: Restriction enzymes PstI and ScaI were employed and the gel image after restriction digest is shown below. The name of the plasmid is Blank 1. The gel lane on the Blank 2 was digested using PstI enzyme. (left or right) The gel lane on the Blank 3 was digested using PstI and ScaI enzymes. (left or right)arrow_forwardExplain how PCR/OLA (polymerase chain reaction/oligonucleotide ligation assay) can be used in the diagnosis of sickle cell disorder . Would you recommend this method for routine diagnosis of sickle cell disorder? Explainarrow_forwardIt is possible to diagnose genetic mutation by PCR. Explainarrow_forward
- what do you think about the ethical impacts of a technology such as crisprarrow_forwardCite three examples of an application of PCR (polymerase chain reaction).arrow_forwardElectro blotting techniques are routinely used in diagnostic microbiology for detection of a specific DNA sequence in the given bacterial culture. Explain.arrow_forward
- For the analysis of plasmid DNA samples, ________ that recognize, bind to and cleave DNA at specific nucleotide sequences are used. Question options: primase restriction enzyme. DNA polymerase I. reverse transcriptase.arrow_forwardWhich of the following ARE part of a typical PCR reaction mixture? DNA ligase dNTPs (mix of nucleoside tri-phosphates) RNA primers made by primase enzyme template DNA, often from cells collected from hair, cheek swab, or blood 2 DNA primers polymerase enzymearrow_forwardDiscuss the principles behind the use of the following techniques in the diagnosis of viral infections, providing examples of viruses diagnosed using these methods: Restriction Fragment Length Polymorphismarrow_forward
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