Essentials of Genetics (9th Edition) - Standalone book
9th Edition
ISBN: 9780134047799
Author: William S. Klug, Michael R. Cummings, Charlotte A. Spencer, Michael A. Palladino
Publisher: PEARSON
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Chapter 10, Problem 13PDQ
Summary Introduction
To review:
The function of the ten subunits of deoxyribonucleic acid (DNA) polymerase III and differences between holoenzyme and the core enzyme.
Introduction:
DNA polymerase III is the primary enzyme complex involved in
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List and describe the function of the ten subunits constituting DNA polymerase III. Distinguish between the holoenzyme and the core enzyme.
Explain the molecular mechanism of DNA polymerization by DNA polymerase and explain why DNA polymerase 3 and not dna polymerase 1 is responsible for replicating the bacterial genome.
Does the addition of a histidine tag affect DNA polymerase activity and or processivity? Give a detailed explanation.
Chapter 10 Solutions
Essentials of Genetics (9th Edition) - Standalone book
Ch. 10 -
CASE STUDY | At loose ends
A researcher was...Ch. 10 - Prob. 2CSCh. 10 - Prob. 3CSCh. 10 - Prob. 4CSCh. 10 - HOW DO WE KNOW? In this chapter, we focused on how...Ch. 10 - Review the Chapter Concepts list on p. 180. These...Ch. 10 - Compare conservative, semiconservative, and...Ch. 10 - Prob. 4PDQCh. 10 - Predict the results of the experiment by Taylor,...Ch. 10 - Prob. 6PDQ
Ch. 10 - Prob. 7PDQCh. 10 - Prob. 8PDQCh. 10 - Prob. 9PDQCh. 10 - Prob. 10PDQCh. 10 - Prob. 11PDQCh. 10 - Prob. 12PDQCh. 10 - Prob. 13PDQCh. 10 -
14. Distinguish between (a) unidirectional and...Ch. 10 - Prob. 15PDQCh. 10 - Define and indicate the significance of (a)...Ch. 10 - Outline the current model for DNA synthesis.Ch. 10 - Why is DNA synthesis expected to be more complex...Ch. 10 - Prob. 19PDQCh. 10 - Several temperature-sensitive mutant strains of E....Ch. 10 - Prob. 21PDQCh. 10 - Prob. 22PDQ
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- Provide a brief summary of the Sanger sequencing method.arrow_forwardDescribe the function of DNA polymerase. Explain why each part of the name DNA polymerase (DNA, polymer, -ase) makes sense.arrow_forwardIn one, simple sentence define the function of the following 1. Helicase = 2. Alpha subunit of DNA polymerase III =arrow_forward
- Considering prokaryotes, what is the enzyme that helps hold DNA polymerase III in place when nucleotides are being added?arrow_forwardDescribe how DNA gyrase causes DNA supercoiling.arrow_forwardName and describe the first four events (and associated enzymes) that happen during DNA replication at the DNA double helix (in correct sequence)arrow_forward
- With regard to DNA replication, define the term bidirectional replication.arrow_forwardExplain the concept & importance of DNA polymerase III ?arrow_forwardSummarize the functions of the following proteins in E. coli DNA replication: DNA polymerase I, DNA polymerase III, DnaA, helicase, SSB, primase, the sliding clamp, clamp loader, DNA ligase, Tus, and topoisomerases.arrow_forward
- Name of a few enzymes involved in DNA replication other than DSNA polymerase and ligase.name the key function each of them?arrow_forwardWhat is an Okazaki fragment? In which strand of replicating DNA are Okazaki fragments found? Based on the properties of DNA polymerase, why is it necessary to make these fragments?arrow_forwardAssume that a cycle of the polymerase chain reaction takes 20 minutes. How many copies of a single DNA fragment will there be after the reaction has been run for six hours?arrow_forward
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